高速逆流色谱分离茶黄素单体的初步研究

Study on Separation of Theaflavins Monomer by High-Speed Countercurrent Chromatography

不同溶剂系统和NaHCO3前处理茶色素复合物对高速逆流色谱(HSCCC)分离茶黄素分离效果比较,以及对仪器参数的优化,探索应用HSCCC分离茶黄素单体。试验结果表明:采用溶剂系统乙酸乙酯、正己烷、甲醇和水(3:1:1:6,V/V)具有较佳的分离效果;NaHCO3前处理明显有助于茶黄素单体的分离;当进样量为200mg、流动相速度1.2ml/min、仪器转速880r/min时,能达到最好的分离效果。

Different solvent systems, and pretreamaent of NaHCO3 to remove the other pigments and the optimized conditions for the equipment have been discussed in the separation of theaflavin monomer by high-speed countercurrent chromatography （HSCCC）. The results have showed that the solvent system of ethyl acetate/hexane/methanol/water （3：1：1：6） could separate the theaflavins and NaHCO3 was helpful to purify the theaflavins. In regards to the equipment parameters the injection amount of tea pigments was 200mg, velocity of mobile phase was 1.2ml/min and the rotate speed was 880r/min.