摘要
草本植物浮萍中提取的多糖类化合物经实验证明具有免疫增强活性.探索浮萍多糖提取物总糖含量的测定方法并对该方法进行评价,同时将其用于浮萍多糖生产制备中的质量控制.采用分光光度法,分别经苯酚-硫酸和蒽酮-硫酸显色,测定浮萍多糖提取物中的总糖含量,并通过线性和范围、精密度、稳定性和准确度等指标评价该方法.实验结果显示苯酚-硫酸法和蒽酮-硫酸法测浮萍多糖所需设备简单、操作快速、结果稳定、灵敏度高,可将其作为浮萍多糖提取物中总糖含量测定的有效方法.
In the course of searching immunodulators from natural herbal sources, the polysaccharide has been isolated from the water extract of duckweed ( Herba Spirodelae seu Lemnae). The authors aim at investigating the methods that deterlnine the content of total saccharides in the mixture, accordingly, to be used as a quality control in the procedure of producing polysaccharides from duckweed. The contents of saccharides in the extraction were mensurated by spertrophotometry of sulphuric acid-phenol and sulphuric acid-anthrone. And the validitiy of methods was evaluated through some indexes such as linearity and range, precision, stability, and accuracy. The results reveal the methods are convenient, rapid, accurate, and high sensitive, thereby leading to a wide and effective use in determining the saccharides contents in polysaccharides extraction from Herba Spirodelae seu Lemnae.
出处
《重庆大学学报(自然科学版)》
EI
CAS
CSCD
北大核心
2005年第10期111-114,共4页
Journal of Chongqing University
关键词
浮萍
多糖
含量测定
评价
Herba Spirodelae seu Lemnae
polysaccharides
content determination
evaluation