壳聚糖醋酸溶液对凝血作用的研究

Research of the Hemostasis Effect of Chitosan Acetic Acid Solution

研究了不同脱乙酰度和不同分子量壳聚糖醋酸溶液的凝血作用.发现壳聚糖醋酸溶液使抗凝血液中红细胞发生了明显的聚集和变形.通过不同分子量和脱乙酰度壳聚糖的促红细胞聚集实验,证明了低脱乙酰度壳聚糖(60%～70%)使红细胞聚集效果更好,分子量在105～106范围内作用不十分明显.对血液的凝血酶时间(TT)、凝血酶原时间(PT)、活化部分促凝血酶原激酶时间(APTT)和纤维蛋白原浓度(FIB)的测定结果验证了壳聚糖醋酸溶液凝血机理不依赖于血小板和常规'瀑布'凝血机制.

The effect of chitosan with different molecular weight and deacetylation degree on blood hemostasis was tested. The experiments found evident alteration of red blood cell morphology and unusual coagglutination between erythrocytes in the anticoagulant blood which was treated by chitosan acetic acid solution. The red blood cells clot formation experiments showed that chitosan with low deacetylation degree（60%-70% ） caused more red blood cells to assemble when compared versus chitosan with other deacetylation degrees. The effect of molecular weight between 10^5 - 10^6 was not obvious. The thrombin time （TT）, prothrombin time （PT）, activated partial thromboplastin time （APTT）, and concentration of fibrinogen （FIB） of blood treated by chitosan acetic acid solution were measured. The results proved that the hemostasis property of chitosan acetic acid solution was independent of the platelets and the normal “Cascade-like” coagulation pathway.