急性脑出血致肺损伤的模型研究及评价

Study and evaluation on model of lung lesion induced by acute cerebral hemorrhage

目的探索建立急性脑出血致肺损伤动物模型的方法,阐释脑源性肺损伤综合征的科学概念,为该综合征的发病机制研究和临床防治奠定基础。方法随机将72只大鼠分为对照组、模型组,2组各分6个亚组(即术前及术后1h、6h、12h、24h、48h),使用Ⅳ型胶原酶—肝素钠—生理盐水联合注射建立脑出血时脑源性肺损伤综合征模型,结合呼吸频率、血气分析、白细胞计数、肺组织含水量、肿瘤坏死因子α(TNFα)及脑、肺组织病理观察,评估该动物模型。结果该模型动物不仅存在明确稳定的脑出血,而且短时间内即出现一系列炎性反应,出现严重的的肺功能损伤和明确的肺组织病理改变。结论该模型可成功地模拟急性脑出血时脑源性肺损伤综合征的病理过程。

Objective To study the method for establishing the animal model of lung lesion induced by acute cerebral hemorrhage, explain the concept of cerebral lung lesion syndrome and lay a foundation for the research of pathogenesis, prevention and treatment of this syndrome. Method To divide randomly 72 rats into the control group and the model group, and then the two groups were redivided into 6 sub-groups respectively（ i. e. the groups of preoperation, postoperation for 1 hour, 6 hours, 12 hours, 24 hours and 48 hours）. The model of cerebral lung lesion syndrome was established by injecting jointly collagenase Ⅳ, heparin sodium and physiological saline. The model was evaluated according to the observation on respiration rate, blood gas analysis, leukocyte count, lung water contents, tumor necrosis factor-or （TNF-a） The model had a clear and stable cerebral he ological changes in the lung tissue ap and the pathological changes in the brain and lung tissues. Results morrhage and a series of inflammation, serious lung lesion and pathin a short time. Conclusion The model can simulate successfully the pathological course of cerebral lung lesion syndrome induced by acute cerebral hemorrhage