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富血小板血浆对牙周膜成纤维细胞损伤模型影响的研究 被引量:2

Effect of Platelet-rich plasma(PRP) on Periodontal Ligament Fibroblasts in an in vitro Wound Healing Model.
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摘要 目的:探讨应用富血小板血浆(PRP)对狗牙周膜成纤维细胞(PDLFs)损伤模型的愈合有无影响。方法:采用组织块法培养狗牙周膜成纤维细胞,取第四代细胞接种于12孔板,待细胞融合后制作直径约8 mm的损伤模型。用含1%PRP和10%PRP的培养液继续培养,于损伤后第1、4、7、9、11天分别检测各组细胞数量及细胞覆盖损伤区的情况。结果:1%PRP和10%PRP均可促进损伤PDLFs的移行和增殖。结论:1%PRP和10%PRP均可促进牙周膜成纤维细胞损伤模型的愈合。 Objective: The aim of this study was to evaluate the influence of platelet - rich plasma (PRP) on cultured periodontal ligament fibroblasts, using an in vitro wound healing model. Methods: Platelet -rich plasma (PRP) was isolated by two - step centrifugation. Primary PDLFs were obtained from mongrel normal first premolar. The 4th passage of PDLFs were cultured in 12 - well chamber plates. The cells were grown to confluence and in vitro wounds were mechanically created, by removing a 8 rnm diameter cells within a circular area using a sterilized silicon bung. The wells were then incubated for 1, 4, 7, 9 and 11 days and the healing speed of these groups was evaluated. Results : The proliferation and the migration of cells were enhanced by addition of 1% PRP and 10% PRP. Conclusion: PRP appears to exert an influence on cells that is compatible with improved wound healing.
出处 《口腔医学研究》 CAS CSCD 2005年第5期504-506,共3页 Journal of Oral Science Research
基金 福建省科技厅重大课题资助项目(编号:2001Z021) 福建省卫生厅青年基金资助项目(编号:2004-1-24)
关键词 富血小板血浆 牙周膜成纤维细胞 生长因子 损伤愈合模型 Platelet - rich plasma (PRP) Periodontal ligament fibroblast (PDLFs) Growth factor Wound - healing model
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参考文献9

  • 1Rincon JC, Haase HR, Bartold PM. Effect of Emdogain on human periodontal fibroblasts in an in vitro wound-healing model [ J ]. J Periodont Res, 2003,38(3): 290-295
  • 2Okuda K, Kawase T, Momose M, et al. Platelet-rich plasma contains high levels of platelet-derived growth factor and transforming growth factor-β and modulates the proliferation of periodontally related cells in vitro [ J]. J Periodontol, 2003,74(6):849-857
  • 3Tischler M. Platelet rich plasma: the use of autologous growth factors to enhance bone and soft tissue grafts [ J ]. N Y State Dent J,2002,68(3 ): 22-24.
  • 4邹忠桃,谭谦.血小板衍化生长因子与创面愈合[J].中华烧伤杂志,2003,19(5):314-317. 被引量:6
  • 5Cho MI, Lin WL, Genco RJ. Platelet-derived growth factormodulated guided tissue regenerative therapy [ J ]. J Periodontol,1995,66(6) :520-530.
  • 6Matsuda N, Lin WL, Kumar NM, et al. Mitogenic, chemotactic,and synthetic responses of rat periodontal ligament fibroblastic cells to polypeptide growth factors in vitro [J]. J Periodontol, 1992,63(6): 515-525.
  • 7Oates TW, Rouse CA, Cochran DL. Mitogenic effects of growth factors on human periodontal ligament cells in vitro [ J ]. J Periodontol, 1993,64(2): 142-148.
  • 8KawaseT, Okuda K, Wolff LF, et al. Platelet-rich plasma-derived fibrin clot formation stimulates collagen synthesis in periodontal ligament and osteoblastic cells in vitro [J]. J Periodontol,2003,74 (6): 858-864.
  • 9李威,闫福华,卢友光,林敏魁,赵欣.富血小板血浆对牙髓成纤维细胞附着、增殖的影响[J].口腔医学研究,2004,20(2):126-128. 被引量:6

二级参考文献38

  • 1汪平,郝建军,史俊南.牛血浆纤维粘连蛋白对人牙髓成纤维细胞增殖、DNA合成、细胞形态的影响[J].牙体牙髓牙周病学杂志,1995,5(2):80-82. 被引量:8
  • 2Kim WJ,Mohan RR. Effect of PDGF, IL- lalpha, and BMP 2/4 on corneal fibroblast chemotaxis:expression of the platelet -derived growth factor System in the cornea. Invest Ophthalmol Vis Sci, 1999,40:1364 -1372.
  • 3Plemons JM, Dill RE, Rees TD, et al. PDGFF - B producing cells and PDGF- B gene expression in normal gingival and cyclosporine A -induced gingival overgrowth. J Periodontol, 1996,67:264 -270.
  • 4Piotrowski J, Majka J,Sano S ,et al. Enhancement in gastric mucosal EGF and PDGF receptor expression with ulcer healing by sulglycotide. Gen Pharmacol, 1995, 26 : 749 - 753.
  • 5Bonomo SR ,Davidson JD,Yu Y ,et al. Hyperbaric oxygen as signal transducer:upregulation of platelet derived growth factor -beta receptor in the presence of HBO2 and PDGF. Undersea Hyperb Med, 1998,25:211 -216.
  • 6Oates TW,Xie JF,Clinton S ,et al. PDGF -alpha recetor subunit expression down - regulated by IL-lbeta in human periodontal ligament cells. J Dent Res,1998,77:1791 - 1798.
  • 7Velhagen KH,Druegg A,Rieck P. Proliferation and wound healing of retionnal pigment epithelium cell in vitro,effect of human thrombocyte concentrate serum and PDGF. Ophthalmoloe, 1999,96:77 - 81.
  • 8Yla OH, Aaltonen V, Bjorkstrand AS, et al. Upregulation of tumor suppressor protein neurofibromin in normal human wound healing and in vitro evidence for platelet derived growth factor (PDGF) and transforming growth factor - betal ( TGF - beta) elicited increase in neurofibromin mRNA steady- state levels in dermal fibroblastgs. J Invest Dermatol, 1998,110:232 - 237.
  • 9Chai Y,Bringas P,Mogharei A,et al. PDGF -A and PDGFR -alpha regulate tooth formation via autocrine mechanism during mandibular morphogenesis in vitro. Dev Dyn, 1998,213:500 -511.
  • 10Andresen JL,Ledetr T,Ehlera N. Keratocyte migration and peptide growth factors:the effect of PDGF, bFGF, EGF, IGF-I, aFGF and TGF-beta on human keratocyte migration in a collagen gel. Curr Eye Res, 1997,16:605 -613.

共引文献10

同被引文献20

  • 1张宇,林野,邱立新,王兴.富血小板血浆促进口腔种植骨再生的临床应用研究[J].中华口腔医学杂志,2004,39(4):269-272. 被引量:44
  • 2孙颖,束蓉,张秀丽,张明珠.TLR4对人牙周膜细胞分泌IL-1β、IL-6和TNF-α的影响[J].上海交通大学学报(医学版),2007,27(6):646-648. 被引量:7
  • 3Yang H,Young D W,Gusovsky F,et al.Cellular events mediated by lipopolysaccharide-stimulated toll-like receptor 4.MD-2 is required for activation of mitogen-activated protein kinases and Elk-1[J].J Biol Chem,2000,275(27):20861-20866.
  • 4Tiranathanaqul S,Pattamapun K,Yongchaitrakul T,et al.MMP-2 activation by actinobacillus actinomycetemcomitans supernatant in human PDL cells was corresponded with reduction of TIMP-2[J].Oral Dis,2004,10(6):383-388.
  • 5Yasuko Y,Takao K,Kenichi S,et al.Inflammatory cytokine gene expression in human ligament fibroblasts stimulated with bacterial lipopolysaccharide[J].Infect Immun,1995,63(9):3576-3581.
  • 6Van Winkelhoff A J,Loos B G,van der Reijden W A,et al.Porphyromonas gingivalis,bacteroides forsythus and other putative periodontal pathogens in subjects with and without periodontal destruction[J].J Clin Periodontol,2002,29(11):1023-1028.
  • 7Wada N,Maeda H,Yoshimine Y,et al.Lipopolysaccharide stimulates expression of osteoprotegerin and receptor activator of NF-kappa B ligand in periodontal ligament fibroblasts through the induction of interleukin-1 beta and tumor necrosis factor-alpha[J].Bone,2004,35(3):629-635.
  • 8Ogura N,Shibata Y,Kamino Y,et al.Stimulation of interleukin-6 production of periodontal ligament cells by Porphyromonas endodontalis lipopolysaccharide[J].Biochem Med Metab Biol,1994,53(2):130-136.
  • 9Holla L I,Fassmann A,Stejskalova A,et al.Analysis of the interleukin-6 gene promoter polymorphisms in Czech patients with chronic periodontitis.J Periodontol,2004,75(1):30-36.
  • 10Aggarwal B B,Sethi G,Nair A,et al.Nuclear factor-kappa B:A holy grail in cancer prevention and therapy[J].Current Signal Trasduction Therapy,2006,1(1):25-52.

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