摘要
用炭凝集试验(CAT)检测呼吸道合胞病毒(RSV),结果表明该法是一种简便、快速、特异的诊断方法。用CAT对16株RSV和8株其它病毒做试验,结果仅RSV凝集,而其它病毒均阴性。用该法与细胞培养法检测83份临床呼吸道感染幼儿鼻咽吸出物,结果CAT法阳性率为69.88%(53/83),细胞培养法为39.75%(33/83),两者阳性检出率相差极显著。阻断试验证明CAT是高度特异的。结果证明CAT具有较高的敏感性与特异性,可用于临床RSV标本的快速检测。
his study reports a simple,rapid and special diagnosis method of carbon agglutination test(CAT)to detect Respiratory Syncytial Virus(RSV).Sixteen specimens of RSV were seen agglu-tination but 8 specimens of other virus were negative.We compared CAT with tissus culture byusing nasopnaryngeal secretions from children with suspected RSV iofection.Of 83 specimens,53(69.88%)were positive by CAT,33(39.75%)were positive by virus culture,There were verysignificant difference in the positive rates beween CAT and culture Blocking test showed thatCAT has high specificity.We believe that CAT has a high sensitivity and specificity and it can beused for the rapid detection of clinical specimens of RSV.
出处
《中国病毒学》
CSCD
1996年第2期116-119,共4页
Virologica Sinica
基金
湖北省科委重点资助课题
关键词
呼吸道合胞病毒
感染
炭凝集试验
诊断
Carbon agglutination test,Monoclonal antibody,Respiratory Syncytial Virus,Anti-gen detection