骨髓增生异常综合征CD34阳性细胞的免疫表型特征

Immunophenotype features of CD34 positive cells in patients with myelodysplastic syndrome

目的探讨骨髓增生异常综合征(MDS)患者CD34阳性(CD34+)细胞免疫表型特征。方法应用一组系列相关单克隆抗体和四色流式细胞术二次设门策略,对20例MDS患者的骨髓细胞悬液CD34+细胞进行免疫表型分析。结果随着MDS的进展[难治性贫血/难治性贫血伴环状铁粒幼细胞→难治性贫血伴原始细胞增多→转化型原始细胞增多性难治性贫血(RA/RAS→RAEB→RAEBT)],在原始细胞群中的CD34+细胞比例逐渐增高(7.6%→40.1%→71.3%),各亚型之间有显著性差异(P<0.05)。在CD45 vs CD34散点图上二次设门取CD34+原始细胞行抗原表达的分析,异常表达HLA-DR及髓系抗原CD13、CD33和CD117、CD15比例减低。CD34在各亚型间有显著性差异(P<0.05)。结论二次设门策略能反映MDS原始细胞的免疫表型特征。

Objective To explore the immunophenotype features of CD34 positive cells in patients with myelodysplastic syndrome （MDS）. Methods The immunophenotypes of the CD34 positive cells in bone marrow nuclear cells of 20 patients with MDS were performed with a panel of monoclonal antibodies and four-color flow cytometry by secondary gating strategy. Results With the progression of MDS from RA/RAS, RAEB to RAEBT, the proportion of CD34＋ cells was gradually increased from 7.6%,40.1% to 71.3% （P〈0. 05）. Using CIN5 vs CD34 gating strategy,the expressions of HLA-DR, CD13, CD33, CD117 on blasts were higher by secondary gating method. However, there were no differences in the expression of above-mentioned antigens on CD34＋ cells in different MDS subtypes （P〈0.05）. Conclusion Secondary gating strategy could accurately reflect the immunophenotype features of blasts in MDS.