小肠杯状细胞在大鼠休克后肠黏膜重建中的作用

Effect of goblet cell in rat intestine on the restitution process of the gut barrier after hemorrhagic shock

目的观察大鼠失血性休克复苏后小肠杯状细胞在肠黏膜重建过程中的作用。方法SD大鼠49只,每只重250～300g,分实验和对照两组,实验组再分为6个组,分别为休克复苏后1、3、6、12、24和36h组;各组留取回肠黏膜,观察休克复苏后早期不同阶段肠黏膜的形态改变、杯状细胞数量变化和肠三叶因子-3(TFF3)的含量。结果休克后肠黏膜明显损伤,3h始肠黏膜出现损伤后修复现象,表现为杯状细胞聚集于损伤的肠黏膜表面;12h后大部分黏膜细胞被杯状细胞覆盖,杯状细胞呈过分泌现象;24h肠黏膜表面细胞连续性得到恢复。黏膜杯状细胞数量24h内从243±13下降到157±9,下降了35.4%(r=-0.910,P<0.01);36h组细胞数量明显回升,但仍低于对照组(t=4.01,P<0.01)。TFF3含量在12h前明显升高,24h有所下降,但仍高于对照组(t=3.24,P<0.05)。结论杯状细胞可能在肠黏膜损伤后的重建过程中起关键作用;TFF3的高表达可能是促进肠黏膜早期重建的重要因素。

Objective To investigate the changes of the goblet cells in the intestine during the restitution process of the gut barrier after hemorrhagic shock. Methods Forty-nine Sprague-Daweley rats with body weight of 250 - 300 g were divided into control group （ n =-7） and experimental group （ n =42）. Rats in the experimental group was further divided into 6 groups （ n =- 7 each） according to different time point at 1, 3, 6, 12, 24, and 36 hours after hemorrhagic shock resuscitation. The specimens from ileum tissue were taken to observe the morphological changes of the intestinal mucosa. The number of goblet cells was determined by light microscope and/or electron microscope. The contents of trefoil factor family 3 （TFF3） of goblet cells were examined using GC-9A gas chromatographic instrument. Results After hemorrhagic shock, mucosal epithelial injury was obvious in the small intestine. Tissue restitution was found after 3 hours, and mostly established after 12 hours. Following tissue restitution, the denuded mucosal surface was covered intensively by goblet cells. The number of goblet cells on the intestinal mucosa was reduced significantly from 243 ±13 at 1 h to 157±9 at 24 h （r =-0.910, P 〈0.01）, and returned to normal level at 36 h. In the experimental group, the content of TFF3 in the intestinal mucosa increased significantly at 12 hours, decreased, but was still higher at 24 hours （ t = 3.24, P 〈 0.05）. Conclusions The goblet cells play a key role in the restitution of intestinal mucosa. High expression of TFF3 may facilitate the intestinal mucosal restitution in the eady phase.