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人参和泼尼松对关木通致大鼠急性肾毒性的干预作用 被引量:6

Intervention effect of ginseng and prednisone on aristolochia manshuriensis induced acute renal toxicity in rats
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摘要 目的:探讨中药人参和泼尼松对关木通所致急性肾毒性的干预作用。方法:54只清洁级雌性SD大鼠随机分组,以关木通煎剂40 g.kg-1.d-1制备急性肾毒性模型。分别给予泼尼松(30 m g.k-g 1.-d 1)和生晒参煎剂(40 g.k-g 1.-d 1)灌胃5 d;第4和8 d测定血、尿生化等指标,免疫组化染色进行肾组织病理学观察;用SPSS11.5软件进行统计学处理。结果:含关木通各组大鼠体重和活动能力下降,而血尿素氮(BUN)和肌酐(C r)、尿N乙酰葡糖胺(NAG)酶、尿2β微球蛋白(2βM G)、转化生长因子β1(TGFβ1)、Ⅳ型胶原均有不同程度增高,其中关木通组差异最大;与单纯关木通组比较,各实验组BUN、C r、尿NAG酶、TGFβ1、Ⅳ型胶原有不同程度的降低。结论:单独用人参或泼尼松与关木通混合灌胃,或同时用人参、泼尼松和关木通混合灌胃,均能减轻关木通对大鼠的急性肾毒性。 Objective: To investigate the intervention effect of ginseng and predinsone on aristolochia manshuriensis -induced acute renal toxicity in rats. Methods: Fifty -four cleaning grade female Sprague- Dawley (SD) rats were randomly divided into five groups. Animal models of acute renal toxicity were established by 40g·kg^-1·d^-1 of aristolochia manshuriensis decoction via gastric tube. Prednisone (30mg·kg^-1·d^-1) and dried raw ginseng decoction (40 g·kg^-1·d^-1) were given via gastric tube for 5 days. On the 4 th and 8 th days after administrating the drug, biochemical indexes of the rats' blood and urine were detected, and immunohistochemical technique was used to estimate the expressions of transforming growth factor-β1 (TGF-β1) and type Ⅳ collagen in the renal tissue of rats. The pathologic changes of kidney were also observed by microscopy. The SPSS 11.5 packages were used to analyze the data. Results: The rats' weight and activity decreased significantly but the levels of blood urea nitrogen (BUN), creatinine (Cr), urine N - acetylglucosaminidase (NAG), urine β2 - microglobulin (β2 - MG), transforming growth factor- β1 (TGF- β1) and collagen-Ⅳ increased significantly in the groups administered with aristolochia manshuriensis, especially in the aristolochia manshuriensis group. The changes of BUN, Cr, urine NAG, TGF - β1 and collagen Ⅳ were attenuated after feeding the rats with prednisone and ginseng compared to those of the rats administered with aristolochia manshuriensis alone. Conclusion: Aristolochia manshuriensisinduced acute renal toxicity in rats could be relieved by ginseng or prednisone alone or combined.
出处 《中国中西医结合急救杂志》 CAS 2005年第5期292-296,共5页 Chinese Journal of Integrated Traditional and Western Medicine in Intensive and Critical Care
基金 河北省中医药管理局科研计划项目(2004100)
关键词 马兜铃酸肾病 人参 关木通 泼尼松 转化生长因子-Β1 Ⅳ型胶原 aristolochic acid nephropathy ginseng aristolochia manshuriensis prednisone transforming growth factor - β1 type Ⅳ collagen
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