黄芩苷对异丙肾上腺素诱导大鼠心肌缺血的治疗作用

Protection effect of baicalin on isoproterenol induced myocardial ischemia in rats

目的:研究黄芩苷对异丙肾上腺素(isoproterenol,ISO)诱导的大鼠心肌缺血的治疗作用及其机理。方法:将45只SD大鼠分为3组,每组15只。正常对照组,不给予任何处理;异丙肾上腺素(ISO)组,皮下注射ISO20mg·kg-1连续2d;黄芩苷组给予ISO后给予黄芩苷治疗。2d后测定其血流动力学指标。并测定血清中的乳酸脱氢酶(LDH)、肌酸磷酸激酶(CK)、肿瘤坏死因子α(TNFα)的含量。用免疫组化测定心肌组织中p38mapk的表达。结果:黄芩苷组较ISO组能明显改善大鼠的心功能,增强心肌的收缩和舒张功能,血清中LDH、CK的含量降低(P<0.01),并能降低血清中TNFα的含量(P<0.01)。心肌中p38mapk的表达量显著降低。结论:黄芩苷对异丙肾上腺素诱导的大鼠心肌缺血具有保护作用,这一保护作用可能是通过下调p38mapk表达从而抑制TNFα的产生有关。

AIM： To study the protection effect of baicalin on isoproterenol （ISO） induced myocardial ischemia in rats and its mechanisms. METHODS： Forty-five Sprague-Dawley rats were randomly divided into 3 groups. The rats in control group （ n = 15） received no treatment. The rats in ISO group （n = 15 ） received two times of subcutaneous injection of ISO （20 mg·kg^-1 ）, which was separated by a 24 hours-interval. The rats in baicalin group （n = 15） received ISO as described above and received baicalin 50mg·kg^-1 ip. simultaneously. Two days later the hemodynamic measurement was performed and the serum levels of LDH, CK and TNF-α were detected. The expression of p38mapk in myocardium was detected by immunohistochemical technique. RESULTS： The heart function of the group treated with baicalin was improved significantly compared with that of control group. The levels of LDH, CK and TNF-α in baicalin group decreased significantly compared with those in control group and the expression of p38mapk in myocardium also decreased markedly. CONCLUSION： Baicalin has protection effect on isoproterenol induced myocardial ischemia in rats, and the effect maybe due to the decrease of TNF-α induced by reducing the p38mapk expression.