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纳米氧化锆碳糊电极固定辣根过氧化物酶用于过氧化氢的测定 被引量:2

An enzymesensor based on strongly negative nano-zirconia-embedded carben paste electrode for H_2O_2 assay
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摘要 辣根过氧化物酶通过与碳糊中纳米氧化锆之间的静电吸附作用而被固定在电极表面,以对苯二酚为电子媒介,通过测定酶催化双氧水产生的还原电流至用于间接测定双氧水的浓度。响应电流与双氧水在3.6×10^(-6)~7.2×10^(-3)mol/L 浓度间呈准线性关系。线性回归方程为:I=8.488×10^(-4)c+0.4892,相关系数为0.9960。 HRP was immobilized on electrode surface involving strong electrostatic absorption interaction between the enzyme and nano - zirconia dioxide. The concentration of H2O2 can be detected by determining the redox current of p - hydroquinone, which was oxidized by H2O2 in the presence of HRP. The linear response to hydrogen peroxide ranged from 3.6 × 10^-6 to 7.2 × 10^-3 mol/L was obtained. The equation can be expressed as: I = 8. 488 × 10^-4 c + 0. 489 2, the correlation coefficient is 0.996 0.
出处 《化学传感器》 CAS 2005年第3期17-20,共4页 Chemical Sensors
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  • 1Topglidis E, Cass A E G, Gilardi G, et al. Protein adsorption on nanocrystalline TiO2 films: an immobilization strategy for bioanalytical devices [J ]. Anal. Chem., 1998,70(23): 5 111 -5 113.
  • 2Topglidis E, Cass A E G, O' Regan B, et al. Immobilisation and bioelectrochemistry of proteins on nanoporous TiO2 and ZnO2 films [J]. J. Electroanal. Chem., 2001,517(1-2): 20-27.
  • 3Cosnier S, Senillou A, Gratzel M, et al. A glucose biosensor based on enzyme entrapment within polypyrrole films electrodeposited on mesoporous titanium dioxide[J]. J. Electroanal. Chem., 1999, 469(2): 176 - 181.
  • 4Klimova T, Ramirez J, CaMeron M, et al. New Mo and NiMo catalysts supported on MCM-41/alumina for thiophene hydrodesulfurization [J]. Stud. Surf. Sci.Catal., 1998, 117 (Mesporous molecular sieves 1998) :493 - 500.
  • 5Wang J, Pamidi P V A, Jing M. Low - potential stable detection of β - NADH at sol - gel derived carbon composite eledtrodes [J]. Anal. Chim. Acta, 1998, 360(1 -3): 171 - 178.
  • 6Dong S, Kuwana T. Activation of glassy carbon electrodes by dispersed metal oxide particales. I. ascorbic acid oxidation[J]. J. Electrochem. Soc., 1984, 131(4):813 -818.
  • 7Oungpipat W, Alexander P, Southwell- Keely P. A reagentless amperometric biosensor for hydrogen peroxide determination based on asparagus tissue and ferrocene mediation [J]. Anal. Chim. Acta, 1995, 309(1-3):35 - 45.

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