摘要
辣根过氧化物酶通过与碳糊中纳米氧化锆之间的静电吸附作用而被固定在电极表面,以对苯二酚为电子媒介,通过测定酶催化双氧水产生的还原电流至用于间接测定双氧水的浓度。响应电流与双氧水在3.6×10^(-6)~7.2×10^(-3)mol/L 浓度间呈准线性关系。线性回归方程为:I=8.488×10^(-4)c+0.4892,相关系数为0.9960。
HRP was immobilized on electrode surface involving strong electrostatic absorption interaction between the enzyme and nano - zirconia dioxide. The concentration of H2O2 can be detected by determining the redox current of p - hydroquinone, which was oxidized by H2O2 in the presence of HRP. The linear response to hydrogen peroxide ranged from 3.6 × 10^-6 to 7.2 × 10^-3 mol/L was obtained. The equation can be expressed as: I = 8. 488 × 10^-4 c + 0. 489 2, the correlation coefficient is 0.996 0.
出处
《化学传感器》
CAS
2005年第3期17-20,共4页
Chemical Sensors