清肝泻火汤对内毒素诱发家兔肝火证的疗效和机理

Study on the Effect and Mechanism of Qinggan Xiehuo Decoction in Treatment of Endotoxininduced Uveitis of Rabbits

采用内毒素诱发家兔肝火证模型，观察了清肝泻火汤的疗效。实验结果表明该方具有以下作用：（１）减轻肝火证临床症状，如皮肤温度、饮水量、呼吸、尿量、易怒、心率等明显缓解（Ｐ＜０．０５或Ｐ＜０．０１）；（２）改善眼部症状，裂隙灯下虹膜充血与房水混浊均显著好转（Ｐ＜０．０１）；（３）减少房水炎性渗出，房水白细胞数与蛋白含量明显降低（Ｐ＜０．０１）。（４）降低血液和房水中炎症介质的浓度（Ｐ＜０．０１，Ｐ＜０．０５）。（５）病理形态学表明，该方具有保护毛细血管和血－房水屏障，减轻眼葡萄膜组织病理损害的作用。

The therapeutic efficacy of Qinggan Xiehuo Decoction (QGXHD)on rabbit model of uveitis induced by endotoxin(EIU)was observed and compared with the control.Results showed that:(1)The symptoms of Liver-Fire such as conjunctival congestion,thirsty dark urine, oliguria,irritability and rapid pulse were markedly relieved(p<0.05～0.01);(2)The ophthalmic signs, iris hyperemia and aqueous humor turbidity observed through slit-lamp were improved significantly(P<0.01);(3)The inflammatory infiltration, the protein content and leukocyte count in the aqueous humor decreased markedly(p<0.01);(4)The concentration of inflammatory mediators in blood and aqueous humor were reduced.(5)Pathological examination showed that the QGXHD could protect the capillary,blood-aqueous barrier and alleviated the uveal damage caused by endotoxin.