摘要
用聚合酶链反应(PCR)产物直接序列分析,测定30例乙型肝炎表面抗原(HBsAg)阳性中国人感染的乙型肝炎病毒S基因“a”决定簇序列,其中adw21例,adr8例,ayw1例。在121到147共27个氨基酸中,121,123~125,127~128,130~133,135~142,144~145和147高度保守,而第126位可为丙氨酸、异亮氨酸或苏氨酸,143位可为丙氨酸、天冬酰胺或苏氨酸,129为谷氨酸,136为酪氨酸各1例。2例HBsAg阴性病人在第123~124间插入2~3个氨基酸,可能是用现行试剂不能检出血清中HBsAg的原因。研究结果说明了中国人感染的HBV“a”决定簇序列的多态性,可为进一步研究HBV的S基因变异提供参照。
The gene encoding “a ” determinant of “ S” antigen from HBsAg positive patients with chronic illness and /or hepatocarcinoma in China was amplified and sequenced directly for its molecular characterization Amino acid residues 122 to 147 of HBsAg were predicated by sequencing the corresponding part of the S gene of HBV DNA in 30 HBsAg-positive sera . Residues were found to be highly-conservative , except at five positions. These were at positions 126. (Ala or Ile or Thr) , 143 (Thr or Ala ) , 129(Glu or Gln),and 136(Ser to Tyr).However ,Sequencing of isolate from two HBsAg negative patients with chronic liver disease revealed insertion mutationts. lnserted sequences introduced 2 or 3 amino acids between codons 122 to 1 2 4 immediately upstream of the ' a ' determinant. Our data might be useful for development of vaccines and diagnostic HBsAg immunoassays,
出处
《中华微生物学和免疫学杂志》
CAS
CSCD
北大核心
1996年第1期1-4,共4页
Chinese Journal of Microbiology and Immunology
基金
本课题受国家自然科学基金资助