高温季节控温诱导池养鳗鲡性腺发育的研究

Induction of pond-cultivated Anguilla japonica gonadial development by controlling temperature in high temperature season

在高温季节(6～9月),采用人工控温的方法,通过注射外源激素hCG和CPE,对日本鳗鲡进行了催熟试验.结果表明,在人工控制温度(18±2℃)条件下,雌性和雄性日本鳗鲡均可达到性成熟,诱导成熟率分别为73.33%和67.77%,而常温条件下(25～32℃)的诱导成熟率分别为0和33.33%.同时对控温条件下诱导成熟的部分雌性鳗鲡进行了催产,其排卵率为70%,受精率为32%,均接近或达到了低温季节相应的指标(平均分别为62.1%和34.2%).对胚胎发育过程也进行了观察,受精卵能正常发育,在22和24℃下,分别经过39 h 15 min和34 h 9 min孵化出膜.试验证明,在高温季节采用人工控温的方法诱导日本鳗鲡性成熟是可能的.

In this study, the pond-cultivated AnguiUa japonica was induced to mature by artificially controlling temperature at 18±2 ℃ in high temperature season （from June to September）, with injection of hCG and CPE. The results showed that both the male and the female eel could be induced to mature, and the artificial maturation induction rate was 73.33% and 67.77%, while under non-controlling temperature 25 ～ 32 ℃, the maturation induction rate was 0 and 33.33 %, respectively. In an ovulation induction test, the matured female eels were induced, with a spawning inducement rate of 70 %, and a fertilization rate of 32 %, which approached or reached the levels in low temperature season （with an average of 62.1% and 34.2 %, respectively）, and the zygote developed normally. At the temperature 22 and 24 ℃, the zygote hatched to fry in about 39 h 15 min and 34 h 9 min. The experiment proved that it was possible to induce the eel to mature by controlling temperature in high temperature season, which provided some theoretical foundations for the techniques of artificially breeding eel in whole year.