蜕皮甾酮对HepG2细胞葡萄糖消耗的影响

Effect of ecdysterone on glucose consumption of HepG2 Cells

目的 探讨蜕皮甾酮体外降糖的作用特点或机制,即能否通过刺激胰岛素分泌而产生降糖作用。方法 检测HepG2细胞24 h培养液中葡萄糖的消耗量;另外测定βTC3细胞胰岛素的释放量。结果 蜕皮甾酮在1×10^-6～10^-4 mol·L^-1浓度范围内可使HepG2细胞的葡萄糖消耗量增加（44%～77%）；蜕皮甾酮的降糖效能随着培养液中葡萄糖浓度的升高而降低；胰岛素对蜕皮甾酮的降糖作用没有明显的影响。蜕皮甾酮没有刺激βTC3细胞胰岛素分泌的作用。结论 蜕皮甾酮可通过肝细胞发挥非胰岛素依赖的降糖作用，但不能刺激胰岛素的分泌。

Aim To investigate whether ecdysterone is able to exert glucose-lowering effect on hepatocytes or stimulate the secretion of insulin. Methods For glucose consumption studies, the amounts of glucose disappeared from the culture medium of HepG2 cells within 24 h were determined. βTC3 cells were also used to monitor insulin secretion. Results The glucose concentrations decreased significantly by ecdysterone 1 × 10^-6- 10^-4 mol · L^-1 ,while glucose consumption increased by 44% -77% with ecdysterone. Glucose consumption declined as it＇s concentrations increased. Insulin had no effect on glucose-lowering of ecdysterone. 15TC3 cells were not stimulated by ecdysterone. Conclusion Ecdysterone is able to exert glucose-lowering effect on hepatocytes which is insulin independent, but has no effect on insulin secretion.