胃癌c－myc，c－erbB－2，EGFR癌基因扩增、共扩增及其临床相关性研究

AMPLIFICATION,CO-AMPLIFICATION OF ONCOGENE c-myc,c-erbB-2,EGFR IN STOMACH CANCER AND IT 'S CLINICAL ASSOSIATION

应用斑点杂交技术（ＤｏｔＢｌｏｔ）分析５０例胃癌中癌基因ｃ－ｍｙｃ，ｃ－ｅｒｂＢ－２．ＦＧＦＲ扩增。结果４４％的胃癌存在至少一个癌基因扩增，其中ｃ－ｍｙｃ扩增率２２％（扩增２～６倍），ｃ－ｅｒｂＢ－２１４％（扩增２～７倍），ＦＧＦＲ１６％（扩增２～９倍）。发现了４例癌基因共扩增。扩增与胃癌临床相关性研究表明，癌基因扩增与肿瘤的恶性程度存在相关性，累积有更多扩增事件的病例预后更为不良。

We have studied the amplification of oncogene c-myc,c-erbB-2,EGFR in 50 cases of gastriccancer by dot blot. The results showed that in 44 % of fifty gastric carcinomas existed amplification of oneof the three oncogenes, The amplification frequency of c-myc was 22%(2-6fold),c-erbB-2 was 14%(2-7fold),EGFR was 16%(2-9fold).Co-amplification of the oncogenes in four cases from this series wasfound. c-myc and c-erbB2 co-amplification were seen in two cases,c-myc and EGFR co-amplification in twocases. The present study showed that the activation of oncogene c-myc,c-erbB-2, EGFR was involved in theprogression of some gastric cancer.The cases with more amplification events may have a poorer prognosis.