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转基因细胞建株工作经验的探讨

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摘要 应用基因转染技术,可将目的基因导入细胞内,通过特定抗生素筛选及扩大培养可获得特殊基因型细胞株。在此建株过程中,采取多种措施及规范的操作预防微生物的污染,要求操作严谨,预防理化性污染及细胞之间交叉的污染。同时做好细胞生长状况的观察及长期冷冻保存工作。
出处 《实用预防医学》 CAS 2005年第5期1229-1231,共3页 Practical Preventive Medicine
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  • 1Wang YR. Heat shock pretreatment prevents hydrogen peroxide injury of pulmonary endothelial cells and macrophages in culture[J].Shock,1996,6(2):134-141.
  • 2Spits DR,Dewey WC,Li GC,Hydrogen peroxide or heat shock induces resistance to hydrogen peroxide in Chinese Hamster fibroblasts[J].J Cell Physiol,1989,131:364-373.
  • 3Jaattela M. Heat shock proteins as cellular lifeguards[J].Ann Med,1999,31:261-271.
  • 4Xiao XZ, Ben jamin IJ.Stress-response proteins in cardiovascular disease[J].Am J Hum Genet, 1999,64(3):685-690.
  • 5Aikawa R, Nitta-Komatsubara Y, Kudoh S, et al. Reactive oxygen species induce cardiomyocyte apoptosis partly through TNF-alpha[J].Cytokine,2002,18(4):179-183.
  • 6Wong HR. Potential protective role of the heat shock response in sepsis[J].New Horiz,1998,6(2):194-200.
  • 7Bhora FY, Wiss RM, Foster AH, et al. Heat shock protects cardiomyocytes from hypoxia-mediated apoptosis by attenuation of nitric oxide[J].In Vivo,2000,14(5):597-602.
  • 8Laity JH, Lee BM, Wright PE. Zinc finger proteins: new insights into structural and functional diversity [J]. Curr Opin Struct Biol , 2001,11(1):39-46.
  • 9Mackay JP. Crossley M. Zinc fingers are sticking together[J].Trends Biochem Sci ,1998, 23(1) :1-4.
  • 10Iuchi S. Three classes of C2H2 zinc finger proteins[J]. Cell Mol Life Sci ,2001, 58(4):625-635.

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