非小细胞肺癌p53、VEGF、MVD的表达及其相关性

The Study of p53、VEGF、MVD Expressions and Their Correlations in Non-small Cell Lung Cancer

目的研究p53、VEGF、MVD在非小细胞肺癌(NSCLC)中的表达及其相关性,探讨其在肺癌的发生、发展中所起的作用,及其与淋巴结转移、血管生成的关系。方法选取外科切除术后联合放疗±化疗的NSCLC病例56例,运用免疫组化方法(SP法)检测术后病理标本中p53、VEGF和MVD的表达,并结合有无淋巴结转移、血管生成,分析三者在NSCLC中的表达及其相关性。结果p53阳性表达率55.36%(31/56),VEGF阳性表达率57.17%(32/56)。MVD平均值为25.80±10.55。MVD值与淋巴结转移呈正相关(P<0.001)。p53、VEGF、MVD两两比较均呈正相关(P<0.001)。结论p53、VEGF、MVD与NSCLC的发生、发展有关。p53、VEGF与MVD正相关,表明p53、VEGF可能参与了调控肿瘤血管生成过程,并有协同作用。MVD与淋巴结转移正相关,提示淋巴结转移与肿瘤血管生长之间可能存在一定的关系。

Objective To study expressions and correlations of p53, VEGF,MVD in NSCLC and discuss their significance in car relations with lymph node metastasis,angiogenesis. Methrcinogenesis and progression of lung cancer, as well as theiods 56 surgically resected patients with NSCLC combined with radiotherapy ± chemotherapy were enrolled in the study group. The expressions of p53, VEGF and MVD were tested by imrnunohistochemical SP method. The results were reciprocally analyzed and compared with lymph node metastasis, angiogenesis. Results Positive expressions of p53, VEGF were found 55.36%（31/56） ,57.17% （32/56） respectively. The mean MVD of the 56 cases was 25.80 ± 10. 55. There was significant positive correlation between MVD and lymph node metastasis （P〈0. 001）. p53, VEGF,MVD had significant positive correlation reciprocally （P〈 0. 001 ）. Conclusion p53, VEGF and MVD had relationship with carcinogenesis and progression of NSCLC. p53,VEGF and MVD had significant positive correlation. It was made clear that p53, VEGF synergistic participated in the regulation of neoangiogenesis process. Significant positive correlation between MVD and lymph node metastasis indicated that there was probably relation between neoangiogenesis and lymph node metastasis, to some extent.