摘要
利用PCR技术从黑曲霉(Aspergillusniger)中扩增并克隆了葡萄糖氧化酶(glucoseoxidase,简称GO)基因,并将马铃薯病原诱导型启动子(Prp1-1基因启动子)与其融合构建了植物表达载体pCAMGO,经农杆菌介导转化获得了转基因草莓。在病原物灰霉菌孢子诱导后,由淀粉-KI的显色反应证实,转基因草莓中GO基因的表达引起H2O2的生成;用草莓灰霉病病原侵染,结果表明:转基因草莓抗灰霉病的能力较对照明显提高。
Glucose oxidase （GO） gene was cloned from Aspergillus niger by PCR. Plant expressing vector was constructed with pathogeny inducing promer （ Prpl-1 gene） and GO gene. GO gene was introduced into strawberry mediated by A grobaterium Tumefociens. Transgenic strawberry released more H2O2 than that of control after induced by spores of graymold; disease resistance of transgenic strawberry was increased more than controlled plants.
出处
《分子植物育种》
CAS
CSCD
2005年第6期797-800,共4页
Molecular Plant Breeding
关键词
GO基因
草莓
遗传转化
抗病
GO gene, Strawberry, Genetic Transformation, Resistance to gray mold
