豚鼠肾阳虚变应性鼻炎模型的建立

Establishment of Guinea Pig Models of Allergic Rhinitis with Kidney-yang De ficiency

目的建立一种新的肾阳虚变应性鼻炎动物模型的方法。方法将45只H artley豚鼠,随机分为4组,即羟基脲+卵清蛋白组、强的松龙+卵清蛋白组、羟基脲+卵清蛋白+肾气汤组、正常对照组。观察各组豚鼠的行为表现,测定试验前后的体重,给药结束后行组胺刺激试验,处死豚鼠作鼻中隔黏膜病理切片观察。结果羟基脲+卵清蛋白组模型同时具有变应性鼻炎(AR)和肾阳虚的特征,该模型比强的松+卵清蛋白组致敏效果好,成模时间短。结论肾阳虚与AR有一定的相关性。用羟基脲+卵清蛋白制作肾阳虚变应性鼻炎豚鼠模型是一种新的可行的方法,为进一步研究变应性鼻炎提供新的途径。

Objective To find a new method for the establishment of allergic rhinitis （AR） models with kidney-yang deficiency. Methods Fourty-five Hartely guinea pigs were randomized into 4 groups： hydroxycarbamide＋ovalbumin （OV）; predisone＋OV; hydroxycarbamide＋OV ＋Shenqi decoction; normal control）. The general behavior and the body weight of guinea pigs were observed before and after treatment. After 3 weeks, the reaction of guinea pigs to histamine nasal drops was observed and then all animals were sacrificed to examine the pathological changes of nasal septum mucosa. Result The new model induced by hydroxycarbamide and OV was proved to be set up successfully： the manifestations of kidney-yang deficiency in the new model were more representative than those induced by predisone and OV, and the time for modeling was shorter. Conclusion Using hydroxycarbamide and OV to set up kedney-yang deficiency AR models is an new feasible method. The result of this study will provide evidence for the further research of kidney-yang deficiency AR.