高效液相色谱法测定复方田七滴丸中人参皂苷Rg_1的含量

Content Determination of Ginsenoside Rg_1 in Fufangtianqi Dropping Pills by HPLC

目的:建立以高效液相色谱法测定复方田七滴丸中人参皂苷 Rg1含量的方法.方法:色谱柱为 Shim-pack VP-ODS,流动相为乙腈-水(25:75),流速为 1.0ml/min,检测波长为 210nm,柱温为 25℃,进样量为 20μ l.结果:人参皂苷 Rg1检测浓度在 37.2～ 186.0μ g/ml范围内线性关系良好(r=0.9 999),平均加样回收率为 101.01%(RSD=0.90%, n=5).结论:本方法简便、灵敏、准确,可用于复方田七滴丸的质量控制.

OBJECTIVE： To establish a HPLC method for content determination of ginsenoside Rg1 in Fufangtianqi dropping pills.METHODS： The separation was performed on a Shim- pack VP- ODS column at the 25℃, the mobile phase was composed of a mixture of acetonitrile- water（25 ： 75）, the flow rate was 1.0 ml/min, the ultraviolet wavelength was 210nm, the sarnplesize was 20μ1 .RESULTS：The linear range of gindenoside Rgl was 37.2-186.0μg/ml（ r = 0.9 999） ,and the average recovery was 101.01% （RSD = 0.90%, n = 5） .CONCLUSIONS： This method is simple, sensitive and accurate, and can be available for the quality control of Fufangtianqi dropping pills,