感染乳鼠组织中克里米亚-刚果出血热病毒的RT-PCR检测与形态学鉴定

RT-PCR detection and morphological identification of CCHF virus in infected suckling mice

目的:检测并鉴定感染组织中的克里米亚-刚果出血热病毒。方法:分别经皮下与腹腔两种途径感染乳小鼠;应用RT-PCR技术对病毒核酸进行检测;利用透射电镜超薄切片技术对病毒进行形态学鉴定。结果:RT-PCR可在心脏、肝脏、脾脏、肾脏以及脑组织内检测到病毒核酸,透射电镜下可在肝细胞胞浆内观察到形态典型的病毒粒子。结论:应用RT-PCR技术可以对感染组织中的克里米亚-刚果出血热病毒进行检测,利用透射电镜可以对病毒的形态进行观察与鉴定。

Objective： To detect and identify Crimean-Congo hemorrhagic fever （CCHF） virus in infected tissues. Methods：The suckling mice were infected with CCHF virus by subcutaneous or intraperitoneal injection, and CCHF virus in the infected tissues was examined by RT-PCR. Morphology of CCHF virus was identified by transmission electron microscopy. Results： The nucleic acid of CCHF virus could be detected in the hearts, livers, spleens, kidneys and brains of the infected suckling mice by the RT-PCR. Virus with typical configuration could be found in the cytoplasm of the hepatocytes under the transmission electron microscope. Conclusion： The virus in infected tissues can be detected by RT-PCR and morphology of CCHF virus can be identified by transmission electron microscopy.