骨髓基质饲养层对精原干细胞体外培养的影响

Study on effect of feeder layers of bone marrow stromal cells on growth of spermatogonial stem cells in vitro

目的以原代培养小鼠骨髓基质细胞为饲养层,探讨精原干细胞能否在该饲养层上生长及生长的影响因素。方法采用原代培养的小鼠骨髓基质细胞,待细胞长成单层后用丝裂霉素C处理并接种生精细胞共培养。结果原代的小鼠培养骨髓基质细胞能很好的维持精原干细胞的非分化性扩增。结论小鼠原代培养的骨髓基质细胞能作为饲养层支持精原干细胞体外非分化性扩增,该细胞有望成为干细胞培养的新的饲养层。

[Objective] To study whether spermatogonial stem cells can grow on the feeder layers of bone marrow stromal cells, and their effecting factors in vitro. [Methods] Mouse bone marrow stromal cells were cultured initially. When the growing cells extended and mitomycin C was added, followed by spermatogonial stem cells which were cultured together. [Results] The feeder layers of bone marrow stromal cells allowed spermatogonial stem cells to proliferate well and remain undifferentiated. [Conclusion] Bone marrow stromal cells enhance spermatogonial stem cell proliferation and undifferentiation as their feeder layers in vitro. Therefore, this maybe become a new feeder layer for stem cell cultures in vitro.