樱桃矮化砧的组培快繁技术研究

On Techniques of Tissue Culture of Cherry Dwarf Rootstick

以中华矮樱、G isela-5、G isela-7为供试材料,进行组培快繁技术研究,结果表明中华矮樱适宜的初代培养基为M S+6-BA 1.0 m g.L-1+NAA 0.1 m g.L-1,增殖培养基为M S+6-BA 1.0 m g.L-1+NAA 0.2 m g.L-1,生根培养基为1/2M S+NAA 0.8 m g.L-1;G isela-5号适宜的初代培养基为M S+6-BA 0.8 m g.L-1+NAA 0.1 m g.L-1,增殖培养基为M S+6-BA 0.8 m g.L-1+NAA 0.2 m g.-L 1,生根培养基为1/2M S+NAA 0.5 m g.-L 1;G isela-7适宜的初代培养基为M S+6-BA 0.8 m g.L-1+NAA 0.1 m g.L-1,增殖培养基为M S+6-BA 0.8 m g.L-1+NAA0.1 m g.-L 1,生根培养基为1/2M S+NAA 0.5 m g.L-1。

Taking Chinese low cherry, Gisela-5 cherry and Gisela-7 cherry as experimental materials, techniques of tissue culture were studied. The results showed that, the most suitable primary culture medium for Chinese low cherry was MS＋6-BA 1.0 mg ·L^-1＋NAA 0.1 mg ·L^-1 ,propagation culture medium is MS＋6-BA 1.0 mg ·L^-1＋NAA 0. 2mg·L^-1, root culture medium 1/2Ms＋NAA 0. 8 mg ·L^-1; the best primary culture medium for Gisela-5 cherry was MS＋6-BA 0. 8 mg·L^-1＋NAA 0. 1 mg ·L^-1 ,propagation culture medium MS＋6-BA 0. 8 mg ·L^-1＋NAA0. 2 mg ·L^-1,root culture was 1/2MS ＋NAA 0. 5 mg ·L^-1; for Gisela-7 cherry, primary culture was MS＋6-BA 0 . 8 mg ·L^-1＋ NAA mg ·L^-1 0. 1mg ·L^-1,propagation culture medium MS＋6-BA 0. 8 mg ·L^-1 ＋NAA 0. 1 mg ·L^-1 ,root culture medium 1/ 2MS＋NAA 0. 5 mg ·L^-1.