持久稳定的大鼠→小鼠造血嵌合体模型的建立

Establishment of stable rat→mouse hematopoietic chimera model

目的建立持久稳定的造血嵌合体模型.方法将预处理后的SD大鼠经尾静脉注射BALB/c小鼠骨髓细胞,诱导形成特异性耐受的嵌合体SD大鼠.30d后,再将此嵌合体大鼠的骨髓反向移植给致死性照射后的BALB/c小鼠,监测小鼠的存活率,移植后30d做皮片移植和混合淋巴细胞培养,观察小鼠嵌合率的变化.结果嵌合体模型小鼠仅有轻度移植物抗宿主病的表现,生存期明显延长,移植后嵌合率较稳定,移植皮片存活期延长,与对照组相比有显著性差异.结论通过输入嵌合体骨髓的方法可建立稳定持久的大鼠→小鼠嵌合体模型.

AIM： To establish a stable animal model of hematopoietic chimerism. METHODS： Conditioned SD rats received bone marrow cells from BALB/c mouse to induce xenogeneic chimeric rats. Thirty days later, lethally irradiated BALB/c mice received bone marrow cells from chimeric rats and their survival rate was moritored daily. Recipient＇s splenic cells were stimulated in mixed lymphocyte culture （MLC） with xenoantigen and skin transplantation was carried out on day 30. RESULTS： Acute graft versus host disease was significantly alleviated, the survival time was prolonged and T cell proliferation was inhibited. More stable xenogeneic chimerism over 90 d was achieved via reverse bone marrow transplantation. CONCLUSION： A long-term xenogeneic chimera model is established by transplanting bone marrow cells of chimeras.