摘要
目的探讨甲壳低聚糖对α-葡萄糖苷酶活性的影响及降糖效果。方法在体外建立标准酶反应体系,分别加入6%甲壳低聚糖50,100,150,200,250,300μl,用4-硝基酚-α-D呋喃葡萄糖苷法测定酶活性;再建立加有6%阿卡波糖200μl的酶反应体系,测定酶活性;以250 mg/(kg.d)剂量灌胃,观察其对实验性糖尿病大鼠空腹及餐后血糖的影响。结果甲壳低聚糖对α-葡萄糖苷酶具有明显抑制作用,抑制效果接近于阿卡波糖;甲壳低聚糖能显著降低糖尿病大鼠空腹及餐后血糖。结论甲壳低聚糖可能具有阿卡波糖样的降血糖作用。
Purpose To study the effects of chitooligosaccharides on α-glucosidase activity and hypoglycemic activity in experimental type 2 diabetic rats. Methods An enzyme-inhibitor model was established to observe inhibition of α-glucosidase activity by chitooligosaccharides or acarbose, chitooligosaccharides or acarbose was given to mice at a dose of 250 mg/（ kg·d）（ ig, for 8 weeks）, and the effect on glucose level of diabeticmice was observed. Results Chitoofigosaccharides could obviously inhibit α-glucosidase activity, and decrease the levels of fasting or pasting glucose. Conclusion This finding suggests that the hypoglycemic effect of chitooligosaccharides is similar to that of acarbose.
出处
《中国生化药物杂志》
CAS
CSCD
2005年第6期327-329,共3页
Chinese Journal of Biochemical Pharmaceutics
基金
湖南省教育厅课题(No.4-02-JY-02C399)
关键词
甲壳低聚糖
Α-葡萄糖苷酶
抑制作用
chitooligosaccharides
α-glucosidase
inhibition
hypoglycemic effect
