改良Hulth造模法复制膝骨性关节炎的实验研究

Experimental Study on Replicating Knee Osteoarthritis by Modified Hulth's Modeling Method

目的探讨复制膝骨性关节炎的实验动物模型。方法用改良Hulth造模法复制膝骨性关节炎,进行X线摄片、透射电镜、关节滑液及血液检测。结果(1)正常组膝关节面平整光滑,细胞及胞膜完整;模型组膝关节内侧间隙明显变窄,关节面粗糙变形,有骨赘,软骨细胞萎缩,胞核固缩,胞膜破损;(2)造模后8周、16周关节滑液内白介素1(IL1)、白介素6(IL6)、肿瘤坏死因子α(TNFα)、基质金属蛋白酶3(MMP3)和血清中两二醛(MDA)、一氧化氮(NO)含量模型组明显升高,16周关节滑膜中超氧化物歧化酶(SOD)活力模型组明显下降,与正常组比较差异有显著性(P<0.01)。结论改良Hulth造模法复制膝骨性关节炎较Hulth法操作简单、创伤小,可保持一定的膝关节稳定性,有利于动物模型复制的成功。

Objective To explore the method of replicating experimental animal model of knee osteoarthritis. Methods Knee osteoarthritis was repicated by modified Hulth＇s modeling method. X-ray photographic and transmission electron microscopic examination, test of the joint synovial fluid of the modeled joint were peformed, and serum contents of malondialdehyde （MDA） and nitric oxide （NO） in blood were measured. Results （1） In the normal control group, the articular surfacc is smooth and glossy, with intact cells and cellular membrane. In the model group, the medial space of the knee joint became obvious narrowed with rough and deformed articular surface and osteophytes, as well as the atrophic chondrocytes with pyknotic cell nucleus and broken ecllular membrane. （2） Eight weeks and 16 weeks after modeling, in the model group, the contents of interleukin-1 （ IL-1）, iuterlcukin-6 （ IL-6 ）, tumor necrosis factor-α （TNF-α） and matrix metalloproteinase-3 （MMP-3） in synovial fluid and the levls of serum MDA and NO were obviously raised, and the activity of superoxide dismutase（SOD） in synovial membrane was obviously lowered 16 weeks after modeling, showing significant difference when compared with those in the normal control group （P〈0.01）, Conclusion Modified Hulth＇s modeling method in replicating knee osteoarthritis is simple in manipulation with less wound, and the condition of modeled knee joint could he maintained stable to certain degree, which is advantageous to the success of animal model replicating.