从黑莓汁加工的废弃物中提取多酚类化合物

Extraction of anthocyanins and polyphenols from blackberry juice-processing waste

黑莓汁加工会产生大量的废弃物黑莓渣,黑莓渣富含多酚类生物活性物质。黑莓渣中总酚含量(以干基计)为6.86%(w/w),花色苷含量(以干基计)为0.38%(w/w)。从黑莓渣中提取多酚和花色苷的研究结果表明,花色苷的最佳提取工艺条件是:以80目的黑莓渣粉为原料,采用含有1%柠檬酸的50%乙醇溶液为提取剂,在35℃提取2次,每次30min、料液比为1∶10。花色苷的提取率为98.33%。总酚的最佳提取工艺条件是:以80目的黑莓渣粉为原料,用含有1%柠檬酸的50%乙醇溶液为提取剂,在55℃提取2次,每次15min、料液比为1∶10,在此条件下总酚的提取率为93.72%。

Blackberry pomaces, the by-product of blackberry processing, were rich in polyphenols. The percentage of total phenol was 6.86%（w/w, on a dry weight basis）, and the total anthocyanin content was 0.38% （w/w, on a dry weight basis）. Extraction of anthocyanins and polyphenols was optimized for yield. It was found that the maximum yield of anthocyanins of 98.33% was obtained when the blackberry pomace powder with 80 mesh in size was extracted twice by 50% ethanol containing 1% citric acid at 35~C at the solid-to- solvent ratio of 1：10 for 30 min. The optimum conditions for extracting total phenols were as below： the blackberry pomace powder with 80 mesh in size was extracted twice by 50% ethanol containing 1% citric acid at 55℃ at the solid-to-solvent ratio of 1：10 for 15 min. Under these conditions, the yield of total phenols was 93.72%.