核酸靶分子与某些中药成分的非共价作用研究

Studies on the Non-covalent Complexes between Oligodeoxynucleotide and Compounds from Some Chinese Herbs

选择与SARS病毒相关的DNA片段作为抗病毒药物筛选的靶分子,利用生物质谱技术,通过对该寡聚去氧核苷酸分子与常见中药黄芪、穿心莲、刺五加、苦杏仁、牛蒡子、栀子、菘蓝(板蓝根、大青叶)及厚朴中的主要化学成分———黄芪甲苷、苦杏仁苷、穿心莲内酯、栀子苷、紫丁香苷、牛蒡子苷、靛蓝、靛玉红、厚朴酚及和厚朴酚的相互作用研究,发现与寡聚去氧核苷酸的作用强度顺序为:黄芪甲苷>苦杏仁苷>穿心莲内酯>栀子苷>紫丁香苷>牛蒡子苷、靛玉红、靛蓝、厚扑酚及和厚朴酚与寡聚去氧核苷酸没有复合物形成。还研究了黄芪甲苷与3种脱氧核苷的相互作用。本方法作为药物筛选具有一定的可行性。

A section of oligodeoxynucleotide （DNA） from SARS virus was selected and synthesized. Its complexes with ten compounds from some Chinese medicine were investigated by negative ion electrospray mass spectrometry. It was found that six compounds can interact with the DNA target molecule. The sequence of interaction intensities andrographolide （ L3 ） 〉 in these compounds is as follows： astragal gardenoside （ L4 ） 〉 syringin （ L5 ） 〉 arctiin oside Ⅳ （L1） 〉 amygdalin （L2） 〉 （L6）. However, indicum （ L7 ）, ndigo （L8 ）, magnolol （L9） and honokiol （L10） did not form any complexes with DNA target molecule in experiments. In this study, the binding intensities between astragaloside Ⅳ （ L1 ） and three nucelosides were also investigated. The above results obtained will be helpful for the screening of anti-virus drugs.