一株产异淀粉酶栖热菌的生长和产酶初步研究

The Growth and the Enzyme Production of A Iso-Amylase-Producing Thermus Strain

异淀粉酶是一类在工业中有重要应用价值的淀粉酶,从云南梁河温泉水样中分离筛选到1株产异淀粉酶的菌株,初步鉴定为栖热菌(Thermus).该菌在8～19 h为对数生长期,17 h生长最快,20 h后进入稳定期,产酶伴随菌体生长同时发生,进入稳定期后产酶迅速提高,39 h产酶量达最高,初始酶活达4.14 u/mL.

Iso-amylase is a kind of amylase of important application value in industry. A strain, initially identified as Thermus sp. according to its morphological and physiological characteristics, was isolated from water sample of Lianghe hot spring in Yunnan. It enters into logarithmic grow phase at 8-9 h, and grows the fastest at 17 h. After enters into stable phase at 20 h its enzyme production raise rapidly, hits to the peak at 39 h. The initial enzyme activity was 4.14μ/mL.