鱼粉中寡肽和游离氨基酸的测定方法

Determination method of oligopeptides and free amino acids in fish meal

采用16％单宁酸沉淀鱼粉中的蛋白质,用凯氏定氮法和甲醛滴定法分别测出滤液中蛋白质和游离氨基酸的含量．并计算出寡肽的含量。结果显示,该方法操作简便,适用于大批量鱼粉中寡肽和游离氨基酸的测定,在测定鱼粉中寡肽和游离氨基酸的含量取得较好结果。

The tannic acid was selected as the precipitant of peptide in fish meal. The contents of protein and free amino acids in the filtrate of fish meal are determined by the Kjeldahe method and formaldehyde-titration method respectively, then calculate the contents of oligopeptides. The results showed that this method was convenient in operation and suitable for application in the determination of oligopeptides and free amino acids in large fish meal samples and the results were good in determination of oligopeptides and free amino acids in fishmeal by using this method.