低分子量灰树花多糖的分离、纯化和抗肿瘤活性

Separation, Purification and Antitumor Activities of Low Molecular Weight Polysaccharide from Grifola frondosa

目的:从灰树花菌丝体中分离纯化低分子量多糖并进行结构鉴定和抗肿瘤活性检测。方法:采用大孔吸附树脂和离子交换纤维素进行分离,SephadexG200柱层析纯化,HPGFC测定分子量,紫外光谱、红外光谱、薄层色谱和高效液相色谱和气相色谱等进行纯度和结构鉴定。用荷瘤小鼠检测抗肿瘤活性。结果:得到大、小两种多糖组分,其中低分子量多糖为白色粉末状,分子量约为2600,单糖组成为D葡萄糖,含α1,3,α1,6,α1,4糖苷键,对S180肉瘤、EAC腹水瘤、Heps实体瘤都有明显抑制作用。结论:分离得到一种灰树花菌丝体多糖,为低分子量的α葡聚糖,初步药效试验表明其具有抗肿瘤作用。

AIM： To separate and purify low molecular weight polysaccharide from Grifola frondosa. METHODS： The polysaccharide was decolored by macroporous resin and separated by Sephadex G200, and examined with UV, IR, TLC, HPLC and GC. RESULTS： A colorless polysaccharide （GFP2） was obtained. MW of GFP2 was only 2600. TLC analysis of the acid hydrolyzed product and GC analysis of the acetylized product showed that the polysaccharide was composed of D-glucose. IR spectrum revealed it contained α-glucosidie bonds. Glucose was linked by α-1, 3, α-1, 6, α-1, 4-glucosidic bonds in GFP2. GFP2 showed some antitumor activity to S180, EAC and HEPS. CONCLUSION： A kind of low MW α-gluean （GFP2） was obtained from Grifola frondosa mycelium and showed antitumor activity.