黄连总生物碱对乙醇致大鼠胃黏膜损伤的保护作用及其机制探讨

Protective effects of total alkaloids from rhizoma Coptis chinensis on alcohol-induced gastric lesion in rats

目的:研究黄连总生物碱对乙醇致大鼠胃黏膜损伤的保护作用,并对其机制进行探讨。方法:观察预先应用黄连总生物碱对乙醇所致的胃黏膜损伤的影响;分析幽门结扎5 h大鼠胃液、胃壁结合黏液和胃腔游离黏液;检测胃黏膜一氧化氮(NO)、丙二醛(MDA)和.OH的含量以及超氧化物歧化酶(SOD)的活力。结果:黄连总生物碱呈剂量依赖性显著抑制乙醇性胃黏膜损伤(P<0.001),其损伤抑制率大于临床常用量的西米替丁,亦大于等量的小檗碱;黄连总生物碱亦显著阻遏乙醇性胃酸分泌,而对胃液量和胃黏液的分泌无明显影响;黄连总生物碱可显著阻遏乙醇性大鼠胃损伤胃黏膜组织内NO含量和SOD活力下降,明显抑制损伤胃黏膜MDA和.OH含量升高。结论:黄连总生物碱有明显抗乙醇性胃黏膜损伤作用。其机制可能与抑制胃酸过度分泌,抑制胃黏膜NO含量下降,阻遏胃黏膜.OH,MDA含量升高以及恢复SOD活力有关,而与胃黏液分泌无关。

Objective： To study the effects of total alkaloids（TA） from rhizoma Coptis chinensis on alcohol-induced gastric lesion in rats and the possible mechanisms. Method： The experimental gastric damges were established by intragastric（ig） absolute ethanol, and possible protective effects of TA given orally previously were evaluated by following parameters： gastric damage indexes, gastric juice volume, acidity, and mucus quantity. The contents of NO, MDA, ·OH, and SOD activity were also measured in gastric mucosa. Result： TA showed significantly inhibitive effects on gastric damages induced by ig ethanol in a dose dependent manner. The effects of TA （ 120 nag· kg^-1 ） were stronger than that of both cimitidine（70 mg·kg^-1） and berberine（100 mg·kg^-1）, the quantity of later was equal to TA as calculated with berbefine. TA significantly suppressed secretion of gastric acid caused by ethanol without clear influences on gastric juice volume and mucus secretion. TA obviously blunted ethanol-induced elevation of MDA and ·OH, as well as decrease of NO level and SOD activity from gastric mucosa. Conclusion： It is suggested that the TA is a potent protective agent against ethanol-induced gastric damages. The mechanism of actions may be related with inhibiting the secretion of gastric acid and blunting the increase of MDA and ·OH,as well as the decrease of NO level and SOD activity from gastric mucus.