转三价抗虫基因棉花遗传特性研究

Genetic characters of transgenic upland cotton cultivars carrying triple genes(Bt+CpTI+GNA)

研究采用农杆菌介导法将外源三价抗虫基因(Bt+CpTI+GNA)导入常规棉花品种中获得转基因再生株,分子检测表明外源基因已在棉花体内表达并遗传给后代材料。其转化再生株后代材料经抗性检测,进行大田选育已至F8代。PCR分子检测与转化的标记基因和外源目的基因抗性三者极有规律性,转化后代材料分离有的符合孟德尔规律,有的则较偏离,其所携带的基因在转基因棉花低代材料中分离规律较为含糊,高代材料则较稳定。

Foreign triple genes（Bt ＋ CpTI ＋ GNA） resistant to cotton insects have been introduced into conventional cotton cultivars by agrobacterium tumefaciens mediated transformation and the regenerated plants have been obtained. The results of molecular detection indicate that the fordgn genes have been transferred into cotton plants. The progeny materb als of transgenic plants are detected by insect resistance. Up to now, the Fs has been got after the selection in the fields. The results of PCR molecular detection, marker genes and foreign target-gene with insect-resistance are quite regular. Some of their progenies＇ segregations coincide with the Mendel＇s regularity of segregation, hut some deviate, and their segregation laws in lower transgenie generation materials are vague while those in high generation materials are stabler.