复方活血补肾中药对尾悬吊大鼠骨质量的影响

Effects of Chinese herbs for blood invigorating and kidney nourishing on bone mass of tail-suspended rats

目的:观察活血补肾中药对尾吊大鼠骨质量的影响。方法:①实验于2004-10-30在航天医学工程研究所动物实验中心完成。选用32只5周龄雄性Wistar大鼠。自制活血补肾中药复方主要成分包括川断、红花等。将大鼠随机分为4组:自由活动组、悬吊组、中药小剂量组、中药大剂量组,每组8只。②自由活动组只进行单笼饲养,大鼠可自由活动;悬吊组与中药组大鼠尾部悬吊,后肢离地,连续悬吊21d;中药小和大剂量组给予活血补肾中药复方(生药)0.1和0.5g/(kg·d)灌胃,悬吊组和自由活动组每日等量生理盐水灌胃,给药21d。③实验结束,采用放射免疫法测血清骨钙素含量。采用三点弯曲实验法测定骨生物力学指标(最大载荷、弹性载荷、最大挠度、弹性挠度)。用称重法观察股骨矿盐含量的变化。④结果用t检验进行统计分析。结果:大鼠32只均进入结果分析。①悬吊组血清骨钙素含量明显低于自由活动组(P<0.01),中药大剂量组血清骨钙素含量明显高于悬吊组(P<0.01),与自由活动组接近。②悬吊组股骨恒重、灰重及骨矿盐含量均明显低于自由活动组(P<0.01),中药大剂量组骨矿盐含量明显高于悬吊组(P<0.05),中药小剂量组与悬吊组差异不明显(P>0.05)。③悬吊大鼠股骨的最大载荷、弹性载荷均明显低于自由活动组(P<0.01)。中药小剂量组、中药大剂量组股骨生物力学指标与悬吊组相比,差异不明显(P>0.05)。结论:①大剂量[0.5g/(kg·d)]活血补肾中药复方有防治骨丢失的作用趋势,但该用量尚不能有效改善力学强度。②小剂量[0.1g/(kg·d)]活血补肾中药复方对失重大鼠骨丢失现象无明显干预效应。

AIM： To investigate the effects of Chinese herbs for blood invigorating and kidney nourishing on bone mass of tail-suspended rats. METHODS： （1） The experiment was completed at Animal Experimental Center of Aerospace Medical Engineering Institute on October 30, 2004, Totally 32 male Wistar rats with 5 weeks 01d were divided randomly into 4 groups： free active group, tall-suspended group and two Chinese herbs groups with high and low dosages, respectively with 8 in each group, Main components of blood invigorating and kidney nourishing compound contained chuangduan and safflower.（2） Rats in free active group were fed in one stage, and rats could move freely; tails of rats in tall-suspended group and Chinese herbs groups were suspended with hindlimb away from the ground for 21 successive days; 0.1 and 0.5 g/（kg·d） blood invigorating and kidney nourishing compound （raw material） were used to perfuse rats in Chinese herbs groups respectively; and rats in tail-suspended group and free active group were perfused with equal volume of saline every day for 21 days. （3） Radio-immunity method was used to assay content of osteoealcin after experiment; three-point bowing method was used to measure index of bone biodynamics （maximal load, elasric load, maximal clearance and elasric clearance）; and weighting method was used to .aeasure content of mineral salt of femur. （4） t test was used to analyze the results. RESULTS： Totally 32 rats entered the final analysis. （1） Content of osteocalcin in tail-suspended group was obviously lower than that in free active group （P 〈 0.01）, but that in high Chinese herbs groups was higher than that in tail-suspended group （P 〈 0.01）, and that was similar to that in free active group. （2） Constant weight and gray weight of femur and content of mineral salt of bone in tail-suspended group were obviously lower than those in free,e active group （P 〈 0.01）; content of mineral salt of bone in high Chinese herbs groups was higher than that in tail-suspended group （P 〈 0.05）; but differences between low Chinese herbs groups and tail-suspended group were not significant （P 〉 0.05）. （3） Maximal load and elastic load of femur in tail-suspended group were lower than those in free active group （P 〈 0.01）, but indexes of biodynamies were not significantly different in Chinese herbs groups as compared with that in tail-suspended group （P 〉 0.05）. CONCLUSION： （1） Blood invigorating and kidney nourishing eompound of high dosage [0.5 g/（kg·d）] has a tendency to prevention and cure of bone loss, but the dosage cannot improve mechanical intensity. （2） Blood invigorating and kidney nt, urishing compound of low dosage [0.1 g/（kg·d）] does not have obvious intervention on bone loss of weightless rats.