果糖二磷酸镁对大鼠心肌缺血再灌注损伤保护作用的剂量依赖性研究

The Dosage Dependence of Cardioprotective Effects of Magnesium Fructose-1,6-diphosphate on Myocardial Ischemia Reperfusion Injury in Rats

目的观察果糖二磷酸镁(FDP-M)对心肌缺血-再灌注损伤过程中血清中CK,LDH,Mg2+浓度、心肌组织内SOD,MDA浓度的影响,探讨果糖二磷酸镁心肌保护作用的机理及其剂量依赖型。方法用整体大鼠急性心肌缺血再灌注损伤模型,结扎冠脉左室前降支40min后,再灌注40min,应用比色法测定血清中CK,LDH,Mg2+含量,采用羟胺法测定心肌组织中SOD含量,采用硫代巴比妥钠(TAB)比色法测定心肌组织内MDA含量。结果与假手术组相比,缺血再灌注损伤组血中CK,LDH浓度显著升高,Mg2+浓度显著降低,组织内MDA含量明显升高,SOD含量明显降低。与缺血损伤组相比,果糖二磷酸镁三个不同剂量保护组血中CK,LDH浓度明显降低,Mg2+浓度明显升高,组织内MDA含量明显降低,SOD含量明显升高,差异具有显著性(P<0.05或P<0.01),此作用均可见明显剂量依赖性。结论果糖二磷酸镁对心肌缺血再灌注损伤有保护作用,并呈明显的剂量依赖型,其保护作用机制与增加组织内SOD含量,减少血中CK,LDH浓度,减少组织内MDA含量有关。

Objective This paper was designed to observe the concentration change of CK, LDH, Mg^2＋ in the blood serum and SOD, MDA in the myocardial to investigate the cardioprotective effect of Magnesium Fructose-1,6-diphosphate during myocardial ischemia reperfusion injury in anesthetized rats. Methods We adopted the model of myocardial ischemia reperfusion injury in anesthetized rats, determined the concentration of CK, LDH and Mg^2＋ in blood serum, and we determined the contents of SOD and MDA in myocardium in the same time. Results The concentration of Mg^2＋ and SOD were decreased and the concentration of CK, LDH and MDA were increased in the ischemia reperfusion group compared with the sham, and concentration of Mg^2＋ and SOD were significantly increased and CK, LDH, MDA contents were decreased in the three groups of Magnesium Fructose-1,6-diphosphate compared with the ischemia reperfusion group. The dosage mentioned above. Conclusions Magnesium Fructose-1,6-dependence was observed of the effect diphosphate has protective effects on myocardial ischemia reperfusion injury. The protective effect may be related to increasing concentration of Mg^2＋ and SOD, relieving CK, LDH,MDA and antilipid peroxidation.