油茶优良无性系子叶体细胞胚植株再生

Plant Regeneration through Somatic Embryogenesis Formation from Cotyledons of Camellia oleifera Clone

以油茶优良无性系‘湘林4号’子叶为外植体,采用附加不同种类激素的MS培养基对其进行组织培养实验。研究结果表明:子叶形成胚性愈伤组织的最适合培养基为MS+2.0mg.L-12,4-D+1.0mg.L-1KT;经胚状体诱导产生不定芽分化的最适合培养基为MS+2.5mg.L-16-BA+1.5mg.L-1IAA;油茶优良无性系的生根培养基以MS+7.0mg.L-1NAA最适;通过对植株再生过程中各阶段的组培材料进行RAPD鉴定分析表明,DNA水平上未发现变异,说明通过组织培养建立的油茶优良无性系再生植株同原无性系无明显差别,最终获得的组培苗木能够保持原无性系的优良特性,其遗传是稳定的。

Cotyledons ofexplants from‘xianglin-4＇ （XL-4）, a Camellia oleifera clone, were cultured in MS medium with different hormones. Results showed that the optimal medium for embryogenetic callus formation was MS with 2,0 mg.L^-1 2,4-D and 1.0 mg.L-1 KT; the optimal medium for shoot development via embryos was MS with 2.5 mg.L^-1 6-BA and 1.5 mg.L^-1 IAA; the optimal medium for root inducement was MS with 7.0 mg.L^-1 NAA. Some materials from different processes ofplantlet regeneration were discriminated by RAPD analysis. However, no mutations or differentiation were discovered. Therefore, it can be concluded that plantlets from tissue culture do not differ from quondam clones and can maintain the hereditary consistency well.