联苯胺对大鼠P53基因损伤作用及器官特异性研究

Study of Rat's p53 Gene Damage and Organ Specificity Induced by Benzidine

目的探讨联苯胺对大鼠作用的主要靶器官及其遗传毒性作用机制。方法将联苯胺腹腔注射染毒SD大鼠,提取肝、肺、肾及膀胱组织的DNA,运用单链探针依赖随机化末端连接物聚合酶链反应(RDPCR)技术检测其对大鼠P53基因外显子7的DNA损伤作用。结果杂交显色后在大鼠肝、膀胱、肺组织检测到了杂交条带,而肾组织未检测到杂交条带。结论联苯胺对大鼠P53基因外显子7具有DNA损伤作用,其致癌机制可能与P53基因损伤有关,联苯胺对大鼠的主要靶器官为肝、膀胱和肺。

Objective Studying the main target organs and the genetic toxicity mechanism of benzidine. Methods SD rats were given benzidine i.p. injection. DNA was extracted from rat＇s liver, kidney, lung and bladders. Then ss probes RDPCR was used to detect the damaged DNA of exon 7 of p53 gene. Results Hybridization bands were found in liver, bladder and lung tissues after ss probes RDPCR, while no hybridization bands were found in kidney tissues. Conclusion The result indicates that benzidine can cause the DNA lesions of exon 7 of p53 gene and its major target organs are liver, bladder and lung. The toxicity mechanism of benzidine is probably related to p53 gene damage.