氟伐他汀对兔心肌缺血再灌注损伤的防治效应

Protective effect of fluvastatin on ischemic reperfused myocardium in rabbits

目的:观察氟伐他汀对心肌缺血再灌注损伤的防治作用及对心肌ICAM-1mRNA表达的影响。方法:24只日本大耳白兔,随机分3组,假手术组、对照组、处理组(给予氟伐他汀10mg·kg-1·d-1喂服1周),所有动物在手术前检测血脂,对照组和处理组复制缺血再灌注模型,假手术组只穿线不结扎。监测血流动力学指标,检测血清乳酸脱氢酶(LDH)和肌酸激酶(CK)的活性。RT-PCR检测缺血区及假手术组对应区域心肌ICAM-1mRNA的表达。结果:各组动物在手术前1d血脂指标无统计学差异;缺血开始后处理组各时点左室舒张末压(LVEDP)小于对照组(P<0·05),左室内压变化最大速率(±dp/dtmax)大于对照组(P<0·05);他汀组LDH-1、CK、CKMB活性均显著小于对照组(均为P<0·01);他汀组心肌组织ICAM-1mRNA表达显著低于对照组(P<0·01),假手术组表达最少。结论:氟伐他汀预处理能减轻心肌缺血再灌注损伤,其机制可能与抑制炎症反应有关。

AIM： To investigate the protective effect of fluvastatin and its influence on ICAM - 1 mRNA expression in ischemia/reperfusion myocardium of normocholesterolemic rabbits. METHODS： 24 rabbits were divided into three groups randomly and myocardial ischemia/reperfusion model in the rabbit was made. Rabbits were subjected to 45 min of regional myocardial ischemia and 2 h of reperfusion. 10 mg· kg^- 1· d^- 1 fluvastatin were administered for one week. Dynamic index of blood flow was recorded and analyzed. Serum activity of CK, CKMB, LDH and LDH - 1 were measured. The expression of ICAM - I mRNA in ischemic myocardium was detected with semi- quantitative RT- PCR. RESULTS： In comparison with control group, pretreatment with fluvastatin decreased LVEDP at the whole observed duration, and spontaneously increased ＋ dp/dtmax. Serum activities of CK, CKMB and LDH- 1 in control group were significantly higher than those in sham group, but heavily reduced in fluvastatin group. Increased expression of ICAM- 1 mRNA due to ischemia reperfusion was reduced significantly in fluvastatin group compare to control group. CONCLUSION： Pretreatment of fluvastatin may reduce inflammation reaction in reperfused myocardium, and this may contribute to its protective effect against experimental myocardial ischemia reperfusion injury.