6种细胞因子对脐血的CFC和LTC-IC的刺激作用

EffectsofSCF,IL┐3,GM┐CSF,G┐CSF,IL┐6andEpoonthecolonyformingcelandlong┐termculture┐initiatingcelinhumanumbilicalcordblood

应用经Ｘ线照射骨髓基质细胞层长期培养法加上６种细胞因子的刺激对脐血中的克隆形成细胞（ＣＦＣ）和长期培养起始细胞（ＬＴＣ－ＩＣ）进行研究。发现脐血中具有丰富的ＣＦＣ和ＬＴＣ－ＩＣ。在不同的细胞因子作用下，ＣＦＣ形成ＣＦＵ－Ｍｉｘ，ＣＦＵ－ＧＭ和ＢＦＵ－Ｅ的能力有不同程度的提高；ＬＴＣ－ＩＣ集落形成率也有明显扩增，尤其ＳＣＦ，ＩＬ－３和ＧＭ－ＣＳＦ的联合应用，更为显著。研究结果还提示：脐血ＬＴＣ－ＩＣ具有与骨髓ＬＴＣ－ＩＣ类似的特征。

Sixgrowthfactors(SCF,IL-3,GM-CSF,G-CSF,IL-6andEpo)wereusedtostimulatecolonyformingcel(CFC)andlong-termculture-initiatingcel(LTC-IC)ofhumanumbilicalcordblood(UCB)inalong-termculturesystem.TheresultsshowedthattherewereanumberofCFCsandLTC-ICsinUCB.ThecapacitiesofCFCforformingCFU-MIX,CFU-GMandCFU-Eandtheplatingefi-ciencyofLTC-ICweregreatlyenhancedunderthestimulationsofthevariouscombinationsofthegrowthfactors,amongwhichthecombinationofSCF,IL-3andGM-CSFwasthemostefective.TheresultsalsoindicatedthatLTC-ICinUCBhadsimilarcharacteristicstothatinhumanbonemarrow.