紫外分光光度法测定灵芝及调脂灵中总灵芝酸的含量

Quantification of Ganoderic Acids in Ganoderma Lucidum and TiaoZhiling by Ultraviolet Spectrometry

目的 建立灵芝和调脂灵中总灵芝酸的含量测定方法。方法 采用紫外分光光度法，以灵芝酸C2为对照品，饱和NaHCO3溶液为溶剂，测定波长260nm。结果 灵芝酸C2在0．0108-0．0486mg·ml^-1范围内呈良好的线性关系，r=0．9995；加样平均回收率为99．2％，RSD为1．76％（n=5）。结论 该法操作简便，灵敏度高，专属性好，重复性好，可以用于灵芝及其凋脂灵的质量控制。

Aim An assay method was developed for determination of ganoderic acids in C.anoderma Lucidum and TiaoZhiling by Ultraviolet Spectrometry. Methods A ganoderic acid C2 standard dissolved in saturated NaHCO3 has a maximum absorbance at 260nm by UV spectrometry. Results The concentration of standard is correlated very well to its optical density at 260nm in the range of 0.010 8 - 0.048 6mg·ml^-1 with a relativity coefficient of 0.999 5.The average recovery was 99.2% （ RSD = 1.76%, n = 5）. Conclusion The above method was easy to use, highly accumt, specific and repetitive, and it should be helpful for monitoring the quality of Ganoderma lucidum material and related products.