摘要
目的观察釉基质蛋白(EMP)对牙周膜细胞(PDLC)在牙周炎患牙牙骨质根面上附着和增殖的影响。方法用EMP处理的牙周炎患牙根片为实验组,用生理盐水处理的患牙根片和健康牙根片分别作为对照。扫描电镜下观察PDLC在根面上的生长状况,用噻唑蓝比色法分析细胞在根片上的附着和增殖。结果实验组与健康对照组根片上,可见细胞间大量丝状伪足相连,优于病变对照组。实验组PDLC的附着和增殖显著多于病变对照组(附着:0·45±0·03与0·37±0·05,P<0·05;增殖:0·71±0·02与0·55±0·08,P<0·01),但低于健康对照组(附着:0·67±0·08,P<0·05;增殖:1·05±0·09,P<0·05)。结论EMP能促进PDLC在牙周炎患牙牙骨质根面上的附着和增殖。
Objective To evaluate the effect of enamel matrix protein (EMP) on the attachment and proliferation of periodontal ligament cells (PDLC) on diseased cementum surfaces in vitro. Methods Cementum chips were obtained from diseased roots exposed to periodontal pocket. Thirteen diseased root cementum chips were conditioned with EMP. Meanwhile, 13 diseased and 13 healthy cementum chips were treated with physiological saline as control. The growth and morphology of PDLC on the root surface were observed after 24 hours incubation by scanning electron microscope (SEM). PDLC attachment and proliferation were quantified using MTr assay at 16 or 72 hours. Results The cells on EMP treated roots under SEM were growing robust like the cells on healthy roots. By contrast, the diseased cementum surface without conditioned with EMP was only partly covered with spindle-shaped cells, with filopodia appearing short and thin. MTT assay indicated that the number of adhered and proliferated cells on diseased cementum chips treated with EMP was significantly greater than that on diseased chips treated with saline (adhesion: 0.45±0.03 vs. 0.37±0.05, P 〈 0.05; proliferation: 0.71±0.02 vs. 0.55±0.08, P 〈 0.01), but less than that on healthy chips (adhesion: 0.45±0.03 vs. 0.67±0.08, P 〈 0.05; proliferation: 0.71±0.02 vs. 1.05±0.09, P 〈 0.05). Conclusions It was suggested that EMP could promote the growth of PDLC on the diseased root cementum surface.
出处
《中华口腔医学杂志》
CAS
CSCD
北大核心
2006年第2期74-76,共3页
Chinese Journal of Stomatology
关键词
牙周炎
牙骨质
显微镜检查
电子
扫描
釉基质蛋白
Periodontitis
Dental cementum
Microscopy, electron, scanning
Enamel matrix proteins