期刊文献+

pH值对Gigaspora margarita孢子萌发、菌丝生长与聚磷酸盐含量的影响 被引量:7

Influence of pH value on the spore germination, hyphal growth and polyphosphate content of Gigaspora margarita
下载PDF
导出
摘要 土壤pH值是影响AM真菌的生理与生态过程的重要因子之一,本试验在培养基上接种Gigasporamargarita的孢子,研究了pH值分别为5.2、6.0和6.8时孢子萌发率、菌丝生长和菌丝中聚磷酸盐(polyP)的含量。结果表明,不同pH条件下的孢子萌发率没有明显差异,培养12d后的萌发率为70%左右;随着pH的升高,菌丝的长度逐渐增加,表明低pH对菌丝的生长有一定的抑制效应;培养12d后,孢子中polyP含量低于菌丝中polyP含量,pH6.0和pH6.8的条件下菌丝中polyP含量明显高于pH5.2的含量,表明低pH也能降低菌丝中的聚磷酸盐含量。认为低pH对菌丝生长和polyP含量的抑制可能是其限制AM真菌功能发挥的重要机制之一。 Soil pH value is one of the important factors affecting the physiological and ecological processes of AM fungi. In the present experiment, spores of Gigaspora margarita were inoculated on media (at pH 5.2, 6.0 and 6.8, respectively), and the spore germination, hyphal growth and polyphosphate (polyP) content in both hyphae and spores, were Investigated. Results showed that no obvious difference in spore germination was observed between different pH. Spore germination rate reached about 70% in 12 days after incubation. Hyphal length gradually increased with the rise in pH value. Indicating an inhibitory effect of low pH on hyphal growth. In 12 days after incubation, polyP content in spores was lower than that in hyphae, and polyP content was clearly higher at pH 6.0 and 6.8 than pH 5.2, suggesting that low pH might decrease the polyp content in hyphae. It is suggested that the inhibition of hyphal growth and polyP content by low pH is one of important mechanisms responsible for the restriction on the functions of AM fungi.
作者 朱红惠 姚青
出处 《菌物学报》 CAS CSCD 北大核心 2006年第1期120-124,共5页 Mycosystema
基金 国家自然科学基金(30370033 30570060) 广东省自然科学基金团队项目(E05202480) 广东省科技计划项目(2004B20501003)资助
关键词 AM真菌 孢子培养 酸性 抑制效应 AM fungi, incubation of spore, acidity, inhibitory effect
  • 引文网络
  • 相关文献

参考文献17

  • 1Ashford A.2002.Tubular vacuoles in arbuscular mycorrhizas.New Phytologist,154 (3):545~547.
  • 2Ault-Riché D,Fraley C,Tzeng C,Kornberg A.1998.Novel assay reveals multiple pathways regulating stress-induced accumulations of inorganic polyphosphate in Escherichia coli.Journal of Bacteriology,80:1841~1847.
  • 3Bécard G,Fortin JA,1988.Early events of vesicular-arbuscular mycorrhiza formation on Ri T-DNA transformed roots.New Phytologist,108:211~218.
  • 4Chiocchio V,Venedikian N,Martinez AE,Menendez A,Ocampo JA,Godeas A,2000.Effect of the fungicide benomyl on spore germination and hyphal length of the arbuscular mycorrhizal fungus Glomus mosseae,Internatl Microbiol,3:173~175.
  • 5Clark RB.1997.Arbuscular mycorrhizal adaptation,spore germination,root colonization,and host plant growth and mineral acquisition at low pH.Plant and Soil,192:15~22.
  • 6Clark RB,Zeto SK.1996a.Growth and root colonization of mycorrhizal maize grown on acid and alkaline soil.Soil Biology and Biochemistry,28 (10/11):1505~1511.
  • 7Clark RB,Zeto SK.1996b.Mineral acquisition by mycorrhizal maize grown on acid and alkaline soil.Soil Biology and Biochemistry,28 (10/11):1495~1503.
  • 8Clark RB,Zeto SK,Zobel RW.1999.Arbuscular mycorrhizal fungal isolate effectiveness on growth and root colonization of Panicum virgatum in acidic soil.Soil Biology and Biochemistry,31:1757~1763.
  • 9Ezawa T,Timothy R.Cavagnaro TR,Smith SE,Smith FA,Ohtomo R.2004.Rapid accumulation of polyphosphate in extraradical hyphae of an arbuscular mycorrhizal fungus as revealed by histochemistry and a polyphosphate kinase/luciferase system.New Phytologist,161 (2):387~390.
  • 10Frey JE,Ellis JR.1997.Relationship of soil properties and soil amendments to response of Glomus intraradices and soybeans.Canadian Journal of Botany Review,75 (3):483~491.

二级参考文献1

共引文献15

同被引文献86

引证文献7

二级引证文献16

;
使用帮助 返回顶部