HPLC测定筋骨草中乙酰哈巴苷的含量

Determination of acetylharpagide in Ajuga decumbens by HPLC

目的：建立筋骨草中乙酰哈巴苷的含量测定方法。方法：采用高效液相色谱法。色谱柱：Phenomenex Luna C18（4．6mm×250mm，5μm）；流动相乙腈-水（15：85）；流速1．0mL·min^-1，检测波长197nm。结果：乙酰哈巴苷含量测定的线性范围为0．6-3．6μm（r=0．9993）。平均加样回收率为99．1％，RSD为2．5％。结论：不同产地的筋骨草中乙酰哈巴苷的含量为0．40％。6．39％；本法简便，灵敏准确，可用于筋骨草的质量控制。

Objective： To establish a method of the quantitative determination of acetylharpagide in Ajuga decumbens. Method： The chromatographic conditions were as follows： a Phenomenex lama C18 column was used, the mobile phase was composed of acetontrile-water （15：85）, the flow rate was 1.0 mL·min^-1 and the UV absorbance detection was set at 197 nm. Result： Linearity of acetylharpagide was in the range of 0.6-3.6μ（ r = 0.999 3）, and the average recovery and RSD were 99.13% and 2.48%, respectively, Conclusion： The contents of acetylharpagide ranged 0.40%-6,39% in A. decumbens, The method was simple, accurate and sensitive.