高效液相色谱法测定金银花药材中3种成分的含量

HPLC determination of three components in Flos Lonicerae

目的:建立高效液相色谱法测定金银花样品中绿原酸、当药苷和忍冬苷的含量。方法:采用 YMC-Pack ODS-C_(18)色谱柱(150mm×4.6mm,5μm),以乙腈(A)-1%冰醋酸水溶液(B)为流动相梯度洗脱,多波长检测绘制金银花药材中绿原酸、当药苷和忍冬苷的 HPLC 图谱。流动相梯度:0min(5%A)→13min(10%A)→30min(25%A)。流速:1.0mL·min^(-1)。波长:0～17min,用325nm 检测绿原酸;17～22min,用237nm 检测当药苷;22～25min 用245nm 检测忍冬苷。柱温:20℃。进样量:15μL。结果:绿原酸、当药苷、忍冬苷3个化合物在测定的范围内均表现出良好的线性(r>0.9999),方法的回收率在101.9%～102.3%之间。结论:该方法操作简便、分离效果好、灵敏度高、重复性好,为较全面控制金银花药材的质量提供了一种可靠的方法。

Objective： To establish an HPLC method for the determination of chlorogenic acid, sweroside and loniceraside in Flos Lonicerae. Methods： An HPLC method was applied with a YMC -Pack ODS -C18（ 150 mm × 4. 6 mm, 5 μm） column by a gradient elution using （A） acetonitrile - （ B ） water （ 1% HAc ） as the mobile phase 0 min （5% A） →13 min （ 10% A） →30 min （25% A）. The flow rate was 1.0 mL · min^-1. The detection was set at UV 325 nm（0→17 min） to detecte chlorogenic acid; UV 237 nm（ 17→22 min） to detecte sweroside; UV 245 nm （22→25 min） to detecte loniceraside respectively. The column temperature was 20 ℃, and injection volume was 15 μL. Results： The recovery of the method was in the range of 101.9% N 102.3%, and the compounds showed good linearity （r 〉 0. 9999） in a detected concentration range. Conclusion： The method is simple, sensitive, accurate and reproducible,it can be used to control the quality of Flos Lonicerae.