荔枝核提取液对糖尿病小鼠模型血糖、血脂等相关指标的干预效应

Interventional effect of litchi nucleus extract fluid on blood glucose, blood lipid and other correlated indexes of diabetic model mice

目的:观察荔枝核提取液对四氧嘧啶糖尿病小鼠血糖、血脂、脑蛋白、脑匀浆谷胱甘肽过氧化物酶、氧自由基含量的影响。方法:实验于2005-04/05在广西右江民族医学院生化教研室实验室完成,本实验室为广西高校重点实验室。①选用健康小白鼠40只,选用10只为正常对照组。对其余30只小鼠进行造模:用四氧嘧啶按200mg/kg剂量用蒸馏水溶解后作一次性腹腔注射,注射后第3天空腹血糖升高稳定后并达8.10mmol/L以上为造模成功,随机将造模成功的小鼠24只分为3个组:荔枝核提取液高、低剂量治疗组,模型组,每组8只。高剂量荔枝核提取液治疗组:每只荔枝核提取液0.3mL灌胃;低剂量荔枝核提取液治疗组用荔枝核提取液0.1mL荔枝核提取液加0.2mL蒸馏水灌胃;1次/d,连续4d。模型组和正常对照组用0.3mL蒸馏水灌胃,1次/d,连续4d。②在造模前、造模后3d,治疗后2,4d各用邻甲苯胺微量法测定空腹血糖。治疗4d后采用GPO-POD酶法测定三酰甘油,采用COD-CE-PAP酶法测定总胆固醇,采用沉淀法测定高密度脂蛋白胆固醇,采用双缩脲法测定脑蛋白,用酶促反应的速度表示脑匀浆谷胱甘肽过氧化物酶的活力,采用α-萘胺法测定脑匀浆氧自由基含量。③计量资料差异比较采用方差分析。结果:四氧嘧啶腹腔注射后有2只小鼠死亡,造模失败4只,最终34只进入结果分析。①空腹血糖:造模前各组小鼠无明显差异(P>0.05);治疗4d后,荔枝核提取液低剂量治疗组和荔枝核提取液高剂量治疗组明显低于模型组(P<0.05),与正常对照组相近(P>0.05)。②治疗4d后血脂:各组三酰甘油差异不明显(P>0.05),荔枝核提取液高剂量治疗组血清高密度脂蛋白胆固醇水平明显高于模型组和荔枝核提取液低剂量治疗组(P<0.05,0.01)。③治疗4d后脑匀浆生化指标:正常对照组、荔枝核提取液高、低剂量治疗组脑谷胱甘肽过氧化物酶活性明显高于模型组(P<0.05),脑氧自由基含量低于模型组(P<0.05)。结论:①荔枝核提取液具有降低四氧嘧啶诱导的糖尿病小鼠血糖水平,调节血脂紊乱状况功能。②荔枝核提取液具有促进氧自由基的清除,增加机体抗氧化能力的作用。

AIM： To observe the effect of the litchi nucleus extract fluid on blood glucose, blood lipid, cerebral protein, brain homogenate, glutathion peroxidase （GSH-Px） and oxygen free radical （O2^-） of diabetic mice induced by alloxan. METHODS： ① The experiment was finished in the Laboratory of Biochemistry, Youjiang Medical College for Nationality from April to May 2005. The laboratory was the key laboratory of Guangxi Zhuang Autonomous Region. Totally 40 mice were selected and were divided into 2 groups： 10 as control group and 30 as model group, Animals in model group were given with 200 mg/kg alloxan after lysis of distilled water. When fasting blood glucose reach to 8.10 mmol/L 3 days after injection, the modeling was successful. Then 24 mice were divided randomly into 3 groups high-dosage, low-dosage treatment group and model group with 8 in each group. In high-dosage group, mice were perfused with 0.3 mL litchi nucleus extract fluid, mice in low-dosage group were perfused with 0.1 mL litchi nucleus extract fluid and 0.2 mL distilled water, once a day for 4 successive days. Mice in model and normal control groups were perfused with 0.3 mL distilled water, once a day for 4 successive days. ②Fasting blood glucose was assayed with toluamide method before and 3 days after modeling and 2 and 4 days ＇after treatment respectively. After 4-day treatment, triglyceride （TG） was assayed with GPO-POD method; total cholesterol （TC） with COD-CE-PAP method; high-density lipoprotein cholesterol （HDL-C） with precipitation titration; protein of brain with biuret method; the activity of gluathion peroxidase in brain homogenate with the speed of enzymatic reaction; content of oxygen free radical with α- naphthylamine. ⑤ Measurement data were compared with analysis of variance. RESULTS： After injection alloxan, 2 mice died, 4 mice failed to model, and totally 34 mice entered the final analysis. ① Fasting blood glucose： There was no significant difference before modeling in each group （P 〉 0.05）; but that in low-dosage and high-dosage groups was lower than that in model group after 4-day treatment, and the value was close to that in normal control group （P 〉 0.05）. ②Blood lipid after 4-day treatment： TC level was not significant difference in each group （P 〉 0.05）; but HDL level in high-dosage groups was higher than that in model group and low-dosage group （P 〈 0.05,0.01）. ③ Biochemical indexes of brain homogenate after 4- day treatment： Level of GSH-PX in normal control group, high-dosage and low-dosage groups was higher that that in model group （P 〈 0.05）, but content oxygen free radical was lower than that in model group （P 〈 0.05）. CONCLUSION：① The litchi nucleus extract fluid can decrease level of blood glucose in diabetic mice induced by alloxan and has the function of regulating the disorder condition of blood lipid. ② The litchi nucleus extract fluid has a certain effect on clearing oxygen free radical and improve anti-oxidation ability.