期刊文献+

重组血管抑制剂CHM-I对骨肉瘤的治疗作用 被引量:2

Therapeutic effect of recombinant angiogenic inhibitor CHM- I on osteosarcoma
下载PDF
导出
摘要 目的:观察重组人软骨调节素(chondromodulin,CHM)蛋白对骨肿瘤的治疗作用。方法:利用RT-PCR技术从人软骨组织中扩增出CHM-I基因片段,并将其克隆到原核表达载体pET28a,在大肠杆菌中诱导表达和纯化重组人软骨调节素蛋白,将重组蛋白与人脐静脉上皮细胞或MG-63骨肉瘤细胞共培养,观察其对内皮细胞形成管样结构和MG-63骨肉瘤细胞生长的影响;将重组蛋白局部注射裸鼠皮下肿瘤,观察其对肿瘤形成的影响。结果:获得纯化的重组人软骨调节素蛋白,该蛋白在体外可以抑制血管内皮细胞管样结构的形成而对肿瘤细胞的生长无影响;重组蛋白瘤内注射可以抑制肿瘤在裸鼠体内的生长。结论:重组人软骨调节素蛋白通过抑制血管形成而抑制肿瘤生长,具有良好的临床应用价值。 Objective: To study the effects of recombinant human Chondromodulin-I protein in treatment of osteosarcoma. Methods: Human Chondromodulin-I gene was amplified from cartilage tissue by RT-PCR and was cloned into procaryotic expression vector Pet28a( + ). The recombinant plasmid Pet(CHM-I) was transformed into E. coli B121 (DE3) ; the product was fused with 6 x His at N-terminal and was purified by Ni2 + 2NTA ion exchange resin. The formation of tube-like cellular networks was assayed by co-incubation of HUVECs and recombinant human Chondromodulin-I. The proliferation of the cells incubated with the recombinant human Chondromodulin-I protein was examined by MTT assay and cell number counter Null mice received local injection of recombinant human Chondromodulin-I protein into the tumors. Results: Rcombinant human CHM-I protein was successfully obtained. The purified CHM-I did not inhibit the proliferation of tumour cells, but inhibited the formation of tubelike cellar network in vitro and tumor growth. Conclusion: Recombitant human Chondromodulin-I protein can inhibit angiogenesis in vitro and growth of tumor in vivo
作者 李琪 夏仁云
出处 《中国肿瘤生物治疗杂志》 CAS CSCD 2006年第1期13-17,共5页 Chinese Journal of Cancer Biotherapy
关键词 软骨调节素 血管生长抑制因子 骨肉瘤 chondromodulin angiogenesis inhibitor osteosarcoma
  • 相关文献

参考文献18

  • 1Gastl G,Hermann T,Steurer M,et al.Angiogenesis as a target for tumor treatment[J].Oncology,1997,54(3):177-184.
  • 2Griscelli F,Li H,Bennaceur GA,et al.Angiostatin gene transfer:Inhibition of tumour growth in vivo by blockage of endothelial cell proliferation associated with a mitosis arrest[J].Proc Natl Acad Sci USA,1998,95(11):6367-6372.
  • 3贾福星,沈先荣,王玲,雷呈祥.软骨抗癌活性成分抑制血管生成机理的研究[J].中国生化药物杂志,1997,18(2):68-71. 被引量:6
  • 4Inoue H,Kondo J,Koike T,et al.Identification of an autocrine chondrocyte colony-stimulating factor:Chondromodulin-Ⅰ stimulates the colony formation of growth plate chondrocytes in agarose culture[J].Biochem Biophys Res Commun,1997,241 (2):395-400.
  • 5Hiraki Y,Tanaka H,Inoue H,et al.Molecular cloning of a new class of cartilage-specific matrix,chondromodulin-Ⅰ,which stimulates growth of cultured chondrocytes[J].Biochem Biophys Res Commun,1991,175(3):971-977.
  • 6Hiraki Y,Inoue H,Iyama K,et al.Identification of chondromodulin I as a novel endothelial cell growth inhibitor.Purification and its localization in the avascular zone of epiphyseal cartilage[J].J Biol Chem,1997,272(51):32419-32426.
  • 7Oshima Y,Sato K,Tashiro F,et al.Anti-angiogenic action of the C-terminal domain of tenomodulin that shares homology with chondromodulin-Ⅰ[J].J Cell Sci,2004,117(Pt 13):2731-2744.
  • 8Hiraki Y,Mitsui K,Endo N,et al.Molecular cloning of human chondromodulin-Ⅰ,a cartilage-derived growth modulating factor,and its expression in Chinese hamster ovary cells[J].Eur J Biochem,1999,260(3):869-878.
  • 9Folkman J.Anti-angiogenesis:New concept for therapy of solid tumors[J].Ann Surg,1972,175(3):409-416.
  • 10Folkman J.Tumor angiogenesis:Therapeutic implications[J].N Engl J Med,1971,285(21):1182-1186.

二级参考文献32

共引文献38

同被引文献31

引证文献2

二级引证文献2

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部