龙柚离体培养快速无性繁殖技术研究

Studies on the Technique of Tissue Culture in vitro and Rapid Propagation of Citrus grandis cv.Long Yu

龙柚的茎尖和幼嫩的茎段离体培养快速繁殖试验研究结果:外植体用75%的乙醇浸泡0.5 min后再用0.1%的HgCl2溶液浸泡10min灭菌效果最好;不同外植体的培养结果是茎段(带腋芽的)的繁殖系数大于茎尖,差异达到显著水平;培养基以MS添加6-BA 0.5 mg/L和IBA 0.2 mg/L对不定芽诱导效果最好,增殖比例达到4.2,与其它处理差异达到极显著;无根苗转入1/2 MS附加IBA 1.0 mg/L的培养基中不定根诱导率为78.3%.添加活性炭对培养材料生根诱导有促进效果.

Citrus grandis Osbeck cv. Long Yu was used in the study Tissue Culture in vitro. The main results were as follows： It was optimal for disinfector by marinating the explants in 75% ethanol 0.5 min and 0.1% HgCl2 10 rnin. The optimal explant was stem with axillary buds. Cultural medium on adventitious shoots induction were investigated. The results showed that MS medium supplemented with 6-BA 0. 5 mg/L and IBA 0.2 mg/L was the best to induce the adventitious shoots. Ingredient of medium ＇for rooting was 1/2 MS supplemented with IBA 1.0 mg/L.