新分离呼肠病毒基因组片段S1全长cDNA克隆及其序列分析

cDNA cloning and sequence analysis of S1 segment of new isolated reovirus strains

目的：克隆测序新分离呼肠病毒的S1全长基因组片段，并对其进行序列分析。方法：从接种呼肠病毒的L929细胞中提取病毒基因组，用改进的单引物扩增技术获得S1片段的全长cDNA克隆井测定其全序列。结果：新分离呼肠病毒的S1片段长1437bp，其核苷酸序列与已知的1—3型呼肠病毒标准株的同源性分另1为59％，61％和48％；推测的cr1序列与标准株的同源性分别为52％，60％和26％。结论：新分离呼肠病毒的S1片段与目前已知的分离株相比有很大差异，有可能是2型呼肠病毒的一个独立分支。

Objective： To clone, sequence and analyze genome segment 1 （S1） of new isolated reovirus strains. Methods： Virus genome was extracted from L929 cell infected with the isolate. Full-length eDNA clone of S1 segment was obtained by improved single primer amplification technique. Nueleotide and amino acid sequences were analyzed using free computer softwares. Results： The total nueleotide length of S1 segment was 1 437 bp. Nueleotide homology with 3 prototypie strains T1L, T2J and T3D was 59% ,61%, and 48%, respectively. Amino acid homology of deduced sigmal protein with 3 prototypie strains was 52%, 60%, and 26%, respectively. Conclusion： S1 segment of new isolated reovirus is remarkably different from that of other known strains, and it might belong to an independent branch of serotype 2 reovirus in evolutionary tree.