山药及其麸炒品的多糖部位对小鼠免疫功能的影响

Comparison of the Effect of Polysaccharides from Rhizoma Dioscoreae and Rhizoma Dioscoreae Preparata on Immune Function in Mice

目的比较生山药及麸炒山药多糖部位对免疫低下小鼠免疫功能调节作用的差异。方法采用碳粒廓清法及血清溶血素试验法,比较生山药及其麸炒品不同提取方法所得的多糖对小鼠非特异性和特异性免疫功能的影响。结果山药经麸炒后冷浸提取的多糖在增加碳粒廓清指数K,增强单核巨噬细胞的吞噬功能及提高溶血素水平方面较生品有更强的作用。结论山药经麸炒后冷浸提取的多糖较生品具有更强的增强细胞免疫和体液免疫的作用,与麸炒山药临床用于补益方剂用法相符合,为进一步阐明麸炒山药的炮制机理奠定了实验基础。

Objective To compare the immunoregulatory role of polysaccharides from Rhizoma Dioscoreae and Rhizoma Dioscoreae Preparata in mice with immunosuppression. Methods Carbon clearance test and hemolysin test were used to evaluate the non - specific and specific immunoregulatory roles of polysaccharides from Rhizoma Dioscoreae and Rhizoma Dioscoreae Preparata by different extraction methods.. Result The polysaccharides extracted with cold water from Rhi- zoma Dioscoreae Preparata had a stronger effect than Rhizoma Dioscoreae on increasing the index of K and enhancing phagocytic function of mononuclear - macrophage and hemolysin level. Conclusion The polysaccharides extracted with cold water from Rhizoma Dioscoreae Preparata have a stronger effect in improving the cellular and humoral immune function than Rhizoma Dioscoreae, which accords with the clinical application and will lay a foundation for further explaining the processing mechanism of Rhizoma Dioscoreae Preparata.